Bifeld Eugenia
Bernhard Nocht Institute for Tropical Medicine, Hamburg, Germany.
altona Diagnostics GmbH, Hamburg, Germany.
Methods Mol Biol. 2019;1971:237-247. doi: 10.1007/978-1-4939-9210-2_12.
Murine bone marrow-derived macrophages (BMMs) can be differentiated within 10 days from ex vivo bone marrow progenitor cells by supplementing the cell growth medium with colony stimulating factor-1 (CSF-1). Mature macrophages express specific myeloid markers which can be labeled and detected by flow cytometry (FACS).BMMs are a valuable tool to investigate the interactions between the Leishmania parasites and their host cell as well as to screen anti-Leishmania components. Options for the readout of in vitro infection experiments are diverse and may range from simple counting of intracellular parasites to the determination of metabolic changes of the intracellular parasite or the infected cell, thus providing the investigator with valuable results.
通过在细胞生长培养基中添加集落刺激因子-1（CSF-1），小鼠骨髓来源的巨噬细胞（BMMs）可在10天内从离体骨髓祖细胞分化而来。成熟巨噬细胞表达特定的髓系标志物，可通过流式细胞术（FACS）进行标记和检测。BMMs是研究利什曼原虫寄生虫与其宿主细胞之间相互作用以及筛选抗利什曼原虫成分的宝贵工具。体外感染实验的读数选项多种多样，可能从简单的细胞内寄生虫计数到细胞内寄生虫或受感染细胞代谢变化的测定，从而为研究人员提供有价值的结果。
