Department of General Surgery, The Fourth Affiliated Hospital, China Medical University, Shenyang, 110000, PR China.
Department of General Surgery, The Fourth Affiliated Hospital, China Medical University, Shenyang, 110000, PR China.
Cancer Lett. 2019 Jul 10;454:120-133. doi: 10.1016/j.canlet.2019.04.005. Epub 2019 Apr 11.
Tumor models have created new avenues for personalized medicine and drug development. A new culture model derived from a three-dimensional system, the tumor organoid, is gradually being used in many fields. An organoid can simulate the physiological structure and function of tissue in situ and maintain the characteristics of tumor cells in vivo, overcoming the disadvantages of traditional experimental tumor models. Organoids can mimic pathological features of tumors and maintain genetic stability, making them suitable for both molecular mechanism studies and pharmacological experiments of clinical transformation. In addition, the application of tumor organoids combined with other technologies, such as liquid biopsy technology, microraft array (MRA), and high-content screening (HCS), for the development of personalized diagnosis and cancer treatment has a promising future. In this review, we introduce the evolution of organoids and discuss their specific application and advantages. We also summarize the characteristics of several tumor organoids culture systems.
肿瘤模型为个性化医学和药物开发开辟了新途径。一种源自三维系统的新型培养模型——肿瘤类器官，正在许多领域逐渐得到应用。类器官可以模拟组织的生理结构和功能，并保持体内肿瘤细胞的特征，克服了传统实验性肿瘤模型的缺点。类器官可以模拟肿瘤的病理特征，并保持遗传稳定性，因此非常适合进行分子机制研究和临床转化的药理学实验。此外，将肿瘤类器官与其他技术（如液体活检技术、微筏阵列（MRA）和高内涵筛选（HCS））相结合，用于开发个性化诊断和癌症治疗具有广阔的前景。在这篇综述中，我们介绍了类器官的发展，并讨论了它们的具体应用和优势。我们还总结了几种肿瘤类器官培养系统的特点。
