Protein dynamics of glycogen phosphorylase.

The glycogen phosphorylase molecule absorbs the ultraviolet energy of a nitrogen laser to form an excited state of the cofactor. The decay rate of this state has a lifetime of 6.7 microseconds, and its sensitivity to bound substrates presents a new perspective of the mechanism. A careful analysis of the decay curve for native enzyme and cofactor analogues showed that the lifetime depends on the conformation of protein groups at the active site and how the residues change with bound substrate. The reactive ternary complexes obtained from either direction of the reaction yielded the same lifetime, indicating a change in the active-site conformation to a common configuration for the cofactor and substrate phosphate. This configuration indicates an increase in the cofactor 5'-PO4 pKa and a possible proton shuttle. The pyridoxal 5'-pyrophosphate reconstituted enzyme showed no conformational change alone or in the presence of oligosaccharide. This result does not support an electrophilic attack by the 5'-PO4 phosphorus.