Le Maréchal P, Froussios C, Azerad R
Biochimie. 1986 Oct-Nov;68(10-11):1211-5. doi: 10.1016/s0300-9084(86)80066-9.
(3R) and (3S) 3-deoxy-3-fluoro-7-phospho-D-arabino hept-2-ulosonic acids (3R and 3S-3F-DAHP) the 3-fluoro analogues of DAHP were synthesized from the corresponding 2-deoxy-2-fluoro hexose-6-phosphates. 3R- and 3S-3F-DAHP were tested as substrates for 3-dehydroquinate synthetase from E. coli. Determination of kinetic parameters showed that their apparent Km and Vm were in the same order of magnitude for these two compounds. Further conversion of 3R- and 3S-3F-DAHP into (6R) and (6S) 6-fluoro dehydroshikimate and (6R) and (6S) 6-fluoro shikimate, respectively, was investigated and results are discussed.
（3R）和（3S）3-脱氧-3-氟-7-磷酸-D-阿拉伯庚-2-酮糖酸（3R和3S-3F-DAHP），即DAHP的3-氟类似物，由相应的2-脱氧-2-氟己糖-6-磷酸合成。对3R-和3S-3F-DAHP作为大肠杆菌3-脱氢奎尼酸合成酶底物进行了测试。动力学参数测定表明，这两种化合物的表观Km和Vm处于相同数量级。分别研究了3R-和3S-3F-DAHP进一步转化为（6R）和（6S）6-氟脱氢莽草酸以及（6R）和（6S）6-氟莽草酸，并对结果进行了讨论。
