State Key Laboratory of Advanced Welding and Joining, and School of Science , Harbin Institute of Technology (Shenzhen) , University Town, Shenzhen 518055 , Guangdong China.
State Key Laboratory of Advanced Welding and Joining, and School of Materials Science and Engineering , Harbin Institute of Technology (Shenzhen) , University Town, Shenzhen 518055 , Guangdong China.
J Phys Chem B. 2019 May 2;123(17):3756-3762. doi: 10.1021/acs.jpcb.9b01941. Epub 2019 Apr 23.
The interaction, including the adsorption and embedding, of a widely applied anticancer drug, doxorubicin, with a lipid membrane was investigated. Second harmonic generation and two photon fluorescence were used as a powerful combination capable in revealing this dynamic process at the interface. The adsorption, association, deassociation and embedding of doxorubicin on the lipid membrane were clearly identified based on the consistency in the dynamic parameters revealed by the time dependent second harmonic generation and two-photon fluorescence measurements. This work also presents a new approach for in situ measurement of the adsorption density of doxorubicin on lipid membrane, benefiting from the two-photon fluorescence signal of doxorubicin being significantly altered by its chemical environment. The analysis of the location and molecular density based on the fluorescent efficiency of the chromophores makes the fluorescence measurement a "surface sensitive" technique as well. The analytical procedures used in this work are expected to aid in understanding the interaction between fluorescent molecules and lipid membranes in general.
研究了一种广泛应用的抗癌药物阿霉素与脂质膜的相互作用，包括吸附和嵌入。二次谐波产生和双光子荧光被用作一种强大的组合，能够在界面处揭示这个动态过程。基于时间相关的二次谐波产生和双光子荧光测量揭示的动态参数的一致性，清楚地识别了阿霉素在脂质膜上的吸附、缔合、解缔和嵌入。这项工作还提出了一种新的原位测量阿霉素在脂质膜上吸附密度的方法，这得益于阿霉素的双光子荧光信号由于其化学环境而发生显著改变。基于发色团的荧光效率对位置和分子密度的分析使荧光测量成为一种“表面敏感”技术。本工作中使用的分析程序有望有助于理解荧光分子与脂质膜之间的相互作用。
