Shenzhen Key Laboratory of Flexible Printed Electronics Technology, School of Science, Harbin Institute of Technology (Shenzhen), University Town, Shenzhen 518055, China.
School of Chemistry and Chemical Engineering, Harbin Institute of Technology, Harbin 150001, China.
Molecules. 2022 Jun 11;27(12):3778. doi: 10.3390/molecules27123778.
Revealing the structures and dynamic behaviors of molecules on lipids is crucial for understanding the mechanism behind the biophysical processes, such as the preparation and application of drug delivery vesicles. Second harmonic generation (SHG) has been developed as a powerful tool to investigate the molecules on various lipid membranes, benefiting from its natural property of interface selectivity, which comes from the principle of even order nonlinear optics. Fluorescence emission, which is in principle not interface selective but varies with the chemical environment where the chromophores locate, can reveal the dynamics of molecules on lipids. In this contribution, we review some examples, which are mainly from our recent works focusing on the application of combined spectroscopic methods, i.e., SHG and two-photon fluorescence (TPF), in studying the dynamic behaviors of several dyes or drugs on lipids and surfactants. This review demonstrates that molecules with both SHG and TPF efficiencies may be used as intrinsic dual-probes in plotting a clear physical picture of their own behaviors, as well as the dynamics of other molecules, on lipid membranes.
揭示脂质上分子的结构和动态行为对于理解生物物理过程背后的机制至关重要，例如药物递送囊泡的制备和应用。二次谐波产生 (SHG) 已发展成为研究各种脂质膜上分子的强大工具，它受益于其界面选择性的固有特性，这源于偶数阶非线性光学的原理。荧光发射在原理上不是界面选择性的，而是随发色团所在的化学环境而变化，它可以揭示脂质上分子的动态。在这篇综述中，我们回顾了一些例子，这些例子主要来自我们最近的工作，重点是结合光谱方法，即 SHG 和双光子荧光 (TPF)，研究几种染料或药物在脂质和表面活性剂上的动态行为。这篇综述表明，具有 SHG 和 TPF 效率的分子可用作内在的双重探针，以清晰地描绘它们自身在脂质膜上以及其他分子的动态行为的物理图像。
