College of Materials Science & Engineering , Zhejiang University of Technology , Hangzhou , Zhejiang 310014 , China.
Langmuir. 2019 May 7;35(18):6120-6128. doi: 10.1021/acs.langmuir.9b00611. Epub 2019 Apr 23.
A dual-sensitive drug delivery system (DDS) based on graphene oxide (GO) which is simultaneously loaded with proapoptotic peptides and anticancer drugs was rationally designed and fabricated for cancer synergetic therapy. Specifically, a kind of cell apoptosis peptide (KLAKLAK) (KLA) was anchored on the surface of GO via a disulfide bond to obtain GO-SS-KLA. Then, the aromatic anticancer drug doxorubicin (DOX) was loaded on GO through π-π conjugation and hydrogen bonding interactions. Finally, bovine serum albumin (BSA) was used to coat the GO carrier to obtain a biological medium-stable GO-based DDS, DOX@GO-SS-KLA/BSA. The results show that KLA and DOX can be released responding to the reductive and pH stimulus inside the cells, respectively, and achieve a synergetic therapy for cancer. Moreover, the results of stability studies show that DOX@GO-SS-KLA/BSA could be stably dispersed in water for more than 8 days and in 10% fetal bovine serum for at least 6 days. The constructed DOX@GO-SS-KLA/BSA exhibits great potential as a drug carrier for co-delivery of various therapeutic agents.
基于氧化石墨烯（GO）的双敏感药物传递系统（DDS），同时负载促凋亡肽和抗癌药物，被合理设计并制备用于癌症协同治疗。具体而言，通过二硫键将一种细胞凋亡肽（KLAKLAK）（KLA）锚定在 GO 的表面上，得到 GO-SS-KLA。然后，通过π-π共轭和氢键相互作用将芳香族抗癌药物阿霉素（DOX）负载在 GO 上。最后，牛血清白蛋白（BSA）用于包覆 GO 载体，得到具有生物中性质稳定的基于 GO 的 DDS，DOX@GO-SS-KLA/BSA。结果表明，KLA 和 DOX 可以分别响应细胞内的还原和 pH 刺激进行释放，从而实现癌症的协同治疗。此外，稳定性研究结果表明，DOX@GO-SS-KLA/BSA 可以在水中稳定分散超过 8 天，在 10%胎牛血清中至少稳定 6 天。所构建的 DOX@GO-SS-KLA/BSA 作为共递输送各种治疗剂的药物载体具有很大的潜力。
