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布氏蟾蜍胚胎发育过程中的腺苷酸核苷酸与能荷

Adenylic nucleotides and energy charge during the embryonic development of Bufo arenarum.

作者信息

Sívori E, Herkovits J, Fink de Cabutti N E

出版信息

Comp Biochem Physiol B. 1986;85(3):573-6. doi: 10.1016/0305-0491(86)90049-0.

DOI:10.1016/0305-0491(86)90049-0
PMID:3098495
Abstract

The contents of ATP, ADP and AMP were determined by HPLC and adenylic energy charge (AEC) was estimated during different stages of the embryonic development of Bufo arenarum up to the tailbud stage. All the developmental stages studied showed a high ATP content (about 1.04-1.48 nmol/emb.). The concentration of ADP was low (0.025-0.041 nmol/emb.) but rose slightly at the neural tube stage. AMP was undetectable before the tailbud stage. AEC values were almost constant (about 0.987-0.992) throughout the period studied. Only a fall at the tailbud stage could be detected which can be related to this more advanced cellular differentiation stage.

摘要

通过高效液相色谱法测定了三磷酸腺苷(ATP)、二磷酸腺苷(ADP)和一磷酸腺苷(AMP)的含量,并在阿根廷蟾蜍胚胎发育直至尾芽期的不同阶段估算了腺苷酸能荷(AEC)。所研究的所有发育阶段均显示出较高的ATP含量(约1.04 - 1.48 nmol/胚胎)。ADP浓度较低(0.025 - 0.041 nmol/胚胎),但在神经管期略有上升。在尾芽期之前未检测到AMP。在所研究的整个时期内,AEC值几乎恒定(约0.987 - 0.992)。仅在尾芽期检测到下降,这可能与这个更高级的细胞分化阶段有关。