种植体周围炎辅助应用釉基质衍生物治疗前后种植体周围液的蛋白质组学研究。
Peri-implant crevicular fluid proteome before and after adjunctive enamel matrix derivative treatment of peri-implantitis.
机构信息
Department of Odontology, Umeå University, Umeå, Sweden.
Department of Molecular Periodontology, Umeå University, Umeå, Sweden.
出版信息
J Clin Periodontol. 2019 Jun;46(6):669-677. doi: 10.1111/jcpe.13108.
AIM
The aim of this study was to explore which peri-implant crevicular fluid (PICF) protein pattern is associated with the active peri-implantitis process.
MATERIALS AND METHODS
Peri-implant crevicular fluid from 25 peri-implantitis sites were subjected to proteomic analysis using liquid chromatography-tandem mass spectrometry before and at 3, 6 and 12 months after treatment, to identify associations between PICF protein pattern and implant loss, bleeding on probing, pocket depth and enamel matrix derivative (EMD) treatment.
RESULTS
Clustering of subjects based on their 3-12 months PICF proteomic profiles by principal component analysis defined two major clusters. Cluster 2 differentiated from cluster 3 by 52 proteins (R = 90%, Q = 80%) and belonging to cluster 2 was associated with implant loss (p = 0.009) and bleeding on probing (p = 0.001). Cluster 3 was associated with implant survival and EMD treatment (p = 0.044).
CONCLUSION
Here, we demonstrate that a specific PICF proteomic profile associates with active peri-implantitis process and implant loss.
目的
本研究旨在探讨哪种种植体周围龈沟液（PICF）蛋白模式与活跃的种植体周围炎过程相关。
材料与方法
在治疗前和治疗后 3、6 和 12 个月，使用液相色谱-串联质谱法对 25 个种植体周围炎部位的种植体周围龈沟液进行蛋白质组学分析，以确定 PICF 蛋白模式与种植体丧失、探诊出血、牙周袋深度和釉基质衍生物（EMD）治疗之间的关联。
结果
通过主成分分析对患者基于其 3-12 个月的 PICF 蛋白质组学图谱进行聚类，定义了两个主要的聚类。聚类 2 通过 52 种蛋白（R = 90%，Q = 80%）与聚类 3 区分开来，属于聚类 2 与种植体丧失（p = 0.009）和探诊出血（p = 0.001）相关。聚类 3 与种植体存活和 EMD 治疗相关（p = 0.044）。
结论
本研究表明，特定的 PICF 蛋白质组学模式与活跃的种植体周围炎过程和种植体丧失相关。
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