比较两种不同海参来源的生物活性岩藻糖基硫酸软骨素的链构象特性。
Comparison of chain conformation properties of bio-active fucosylated chondroitin sulfates from two different sea cucumbers.
机构信息
Weifang Medical University, Weifang 261053, Shandong, China; Weifang Key Laboratory for Food Nutrition and Safety, Weifang 261053, Shandong, China.
Central Hospital of Zibo, Zibo 255000, Shandong, China.
出版信息
Int J Biol Macromol. 2019 Jul 15;133:44-50. doi: 10.1016/j.ijbiomac.2019.04.080. Epub 2019 Apr 12.
The present studies aimed to investigate the chain conformation of fucosylated chondroitin sulfates from two different sea cucumbers Apostichopus japonicus (fCS-Aj), and Holothuria mexicana (fCS-Hm). The chain conformations were determined by SEC-MALLS-Visc-RI, indicating that the chain conformations of fCS-Aj and fCS-Hm were random coil and extended linear chain structure in PBS, respectively. Our results suggested that the formation of linear chain of fCS were due to a synergistic effect of molecular weight, sulfate content and sulfation patterns. The worm-like cylinder model indicated that fCS-Aj possessed more flexible chains compared with fCS-Hm. fCS-Aj and fCS-Hm all exhibited as negatively charged polymers with good thermal stability. The present studies were important for understanding the structure-activities relationship of fCSs.
本研究旨在研究两种不同海参（刺参（fCS-Aj）和墨西哥海参（fCS-Hm））来源的岩藻糖基硫酸软骨素的链构象。通过 SEC-MALLS-Visc-RI 确定了链构象，结果表明 fCS-Aj 和 fCS-Hm 在 PBS 中的链构象分别为无规卷曲和伸展线性链结构。我们的结果表明，fCS 线性链的形成归因于分子量、硫酸根含量和硫酸化模式的协同作用。蠕虫状圆柱模型表明，fCS-Aj 与 fCS-Hm 相比，具有更灵活的链。fCS-Aj 和 fCS-Hm 均表现为带有良好热稳定性的带负电荷聚合物。本研究对于理解 fCSs 的结构-活性关系具有重要意义。
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