Zhu Zhenjun, Zhu Beiwei, Ai Chunqing, Lu Jiaojiao, Wu Sufeng, Liu Yili, Wang Linlin, Yang Jingfeng, Song Shuang, Liu Xiaoling
College of Light Industry and Food Engineering, Guangxi University, Nanning, 530004, China; School of Food Science and Technology, National Engineering Research Center of Seafood, Dalian Polytechnic University, Dalian, 116034, China; National & Local Joint Engineering Laboratory for Marine Bioactive Polysaccharide Development and Application, Dalian, 116034, China.
School of Food Science and Technology, National Engineering Research Center of Seafood, Dalian Polytechnic University, Dalian, 116034, China; National & Local Joint Engineering Laboratory for Marine Bioactive Polysaccharide Development and Application, Dalian, 116034, China.
Carbohydr Res. 2018 Aug;466:11-17. doi: 10.1016/j.carres.2018.07.001. Epub 2018 Jul 3.
Fucosylated chondroitin sulfate (FCS) and fucoidan (FUC) are two main bioactive polysaccharides in sea cucumbers. A novel method for quantitation of FCS and FUC was developed by detecting chondroitin disaccharide and fucose produced through acid hydrolysis using HPLC-MS/MS. The present method showed satisfactory performance for both saccharides. It was applied to assay sea cucumbers (Stichopus japonicus) reared in pond grow-out or bottom sowing, and the results were compared with those obtained by traditional HPLC method and 1,9-dimethylmethylene blue test, which could only provide the total sea cucumber polysaccharide (SCP) contents. No difference of total SCP content was observed between sea cucumbers reared through different ways, while a higher ratio of FCS to FUC of sea cucumbers of pond grow-out was revealed by the present method. Thus, this novel method is potential to quantify the two polysaccharides and could be a powerful tool for quality evaluation of sea cucumbers.
岩藻糖基化硫酸软骨素（FCS）和岩藻聚糖（FUC）是海参中的两种主要生物活性多糖。通过使用HPLC-MS/MS检测酸水解产生的软骨素二糖和岩藻糖，开发了一种定量FCS和FUC的新方法。该方法对两种糖类均表现出令人满意的性能。将其应用于池塘养殖或底播养殖的海参（刺参）的测定，并将结果与传统HPLC方法和1,9-二甲基亚甲基蓝试验获得的结果进行比较，后两者只能提供海参总多糖（SCP）含量。通过不同方式养殖的海参之间未观察到总SCP含量的差异，而本方法显示池塘养殖海参的FCS与FUC比例更高。因此，这种新方法具有定量这两种多糖的潜力，并且可能成为海参质量评估的有力工具。
