基于 CRISPR/Cas9 的聚合物纳米颗粒治疗/抑制微生物感染。
A CRISPR/Cas9 based polymeric nanoparticles to treat/inhibit microbial infections.
机构信息
Center for Nanobiotechnology Research, Department of Biological Sciences, Alabama State University, Montgomery, AL, 36104, USA.
Amity Institute of Biotechnology, Amity University, Jaipur, Rajasthan, 303002, India.
出版信息
Semin Cell Dev Biol. 2019 Dec;96:44-52. doi: 10.1016/j.semcdb.2019.04.007. Epub 2019 May 23.
The latest breakthrough towards the adequate and decisive methods of gene editing tools provided by CRISPR/Cas9 (Clustered Regularly Interspaced Short Palindromic Repeat/CRISPR Associated System), has been repurposed into a tool for genetically engineering eukaryotic cells and now considered as the major innovation in gene-related disorders. Nanotechnology has provided an alternate way to overcome the conventional problems where methods to deliver therapeutic agents have failed. The use of nanotechnology has the potential to safe-side the CRISPR/Cas9 components delivery by using customized polymeric nanoparticles for safety and efficacy. The pairing of two (CRISPR/Cas9 and nanotechnology) has the potential for opening new avenues in therapeutic use. In this review, we will discuss the most recent advances in developing nanoparticle-based CRISPR/Cas9 gene editing cargo delivery with a focus on several polymeric nanoparticles including fabrication proposals to combat microbial infections.
最新的突破是利用 CRISPR/Cas9（成簇规律间隔短回文重复/CRISPR 相关系统）提供的适当且决定性的基因编辑工具方法，已被重新用作基因工程真核细胞的工具，现在被认为是基因相关疾病的主要创新。纳米技术提供了一种替代方法来克服传统问题，其中输送治疗剂的方法已经失败。使用纳米技术有可能通过使用定制的聚合物纳米粒子来安全有效地输送 CRISPR/Cas9 组件。将两者（CRISPR/Cas9 和纳米技术）结合起来，有可能开辟治疗用途的新途径。在这篇综述中，我们将讨论基于纳米粒子的 CRISPR/Cas9 基因编辑货物输送的最新进展，重点介绍几种聚合物纳米粒子，包括制造建议，以对抗微生物感染。
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