激动剂诱导的内皮细胞 Ca 信号微区。

Agonist-evoked endothelial Ca signalling microdomains.

机构信息

Physiology, School of Medical Sciences, University of New South Wales, Sydney, NSW 2052 Australia.

Physiology, School of Medical Sciences, University of New South Wales, Sydney, NSW 2052 Australia; Biomedical Science, School of Health and Sports Science, University of the Sunshine Coast, Maroochydore, Qld 4558 Australia.

出版信息

Curr Opin Pharmacol. 2019 Apr;45:8-15. doi: 10.1016/j.coph.2019.03.005. Epub 2019 Apr 12.

DOI:10.1016/j.coph.2019.03.005

PMID:30986569

Abstract

Localized, oscillating Ca signals have been identified in discrete microdomains of vascular endothelial cells. At myoendothelial contacts (between endothelial and smooth muscle cells), both endothelial Ca pulsars (IP-mediated release of intracellular Ca) and Ca sparklets (extracellular Ca entry via TRP channels) contribute to endothelium-dependent hyperpolarization of smooth muscle, vasodilation, and feedback control of vasoconstriction. Ca sparklets occurring at close-contact domains between endothelial cells are possibly involved in conducted hyperpolarization and spreading vasodilation in arterial networks. This review summarizes these Ca signalling phenomena, examines the proposed mechanisms leading to their generation by G-protein-coupled receptor agonists, and explores the proposed physiological roles of these localized and specialized Ca signals.

摘要

局部、振荡的 Ca 信号已在血管内皮细胞的离散微域中被识别。在肌内皮连接处（内皮细胞和平滑肌细胞之间），内皮细胞 Ca 脉冲（IP 介导的细胞内 Ca 释放）和 Ca 闪烁（通过 TRP 通道的细胞外 Ca 内流）都有助于平滑肌的内皮依赖性超极化、血管舒张以及血管收缩的反馈控制。发生在内皮细胞之间紧密接触域的 Ca 闪烁可能参与动脉网络中的传导性超极化和扩散性血管舒张。本综述总结了这些 Ca 信号现象，考察了由 G 蛋白偶联受体激动剂引起这些信号产生的所提出的机制，并探讨了这些局部和专门化 Ca 信号的拟生理作用。