Department of Aquaculture, Institute of Aquaculture and Environmental Safety, Faculty of Agricultural and Environmental Sciences, Szent István University, 1. Páter Károly St., H-2100, Gödöllő, Hungary.
Department of Aquaculture, Institute of Aquaculture and Environmental Safety, Faculty of Agricultural and Environmental Sciences, Szent István University, 1. Páter Károly St., H-2100, Gödöllő, Hungary.
Chemosphere. 2019 Jul;227:151-161. doi: 10.1016/j.chemosphere.2019.04.014. Epub 2019 Apr 8.
The use of microinjection of newly fertilized zebrafish eggs as an appropriate tool for qualifying the biodetoxification properties of toxin-degrading microbes was investigated. Ochratoxin A (OTA), bacterial degradation products of OTA and bacterial metabolites of the Cupriavidus basilensis ŐR16 strain were microinjected. Results showed that variations in the injected droplet size, and thus treatment concentrations, stayed within ±20%, moreover embryo mortality did not exceed 10% in controls, that is in accordance with the recommendations of the OECD 236 guideline. The highest lethality was caused by OTA with a significantly higher toxicity than that of bacterial metabolites or OTA degradation products. However, toxicity of the latter two did not differ statistically from each other showing that the observed mortality was due to the intrinsic toxicity of bacterial metabolites (and not OTA degradation products), thus, the strain effectively degrades OTA to nontoxic products. Sublethal symptoms also confirmed this finding. RESULTS: confirmed that microinjection of zebrafish embryos could be a reliable tool for testing the toxin-degrading properties of microbes. The method also allows comparisons among microbial strains able to degrade the same toxin, helping the selection of effective and environmentally safe microbial strains for the biodetoxification of mycotoxins in large scale.
研究了将新受精的斑马鱼卵的微注射用作定性研究毒素降解微生物的生物解毒特性的合适工具。将赭曲霉毒素 A (OTA)、OTA 的细菌降解产物和恶臭假单胞菌 ŐR16 菌株的细菌代谢物进行微注射。结果表明，注射液滴大小的变化（即处理浓度的变化）保持在±20%以内，并且在对照组（即符合 OECD 236 指南建议）中胚胎死亡率不超过 10%。OTA 的致死率最高，其毒性明显高于细菌代谢物或 OTA 降解产物。然而，后两者的毒性没有统计学差异，表明观察到的死亡率是由于细菌代谢物（而不是 OTA 降解产物）的固有毒性所致，因此，该菌株有效地将 OTA 降解为无毒产物。亚致死症状也证实了这一发现。结果证实，斑马鱼胚胎的微注射可以成为测试微生物降解毒素特性的可靠工具。该方法还允许对能够降解相同毒素的微生物菌株进行比较，有助于选择有效且环境安全的微生物菌株，以便在大规模范围内对霉菌毒素进行生物解毒。
