Almasio P L, Hughes R D, Williams R
Hepatology. 1986 Nov-Dec;6(6):1340-5. doi: 10.1002/hep.1840060620.
The plasma levels of the opsonic glycoprotein fibronectin are decreased in patients with fulminant hepatic failure, which may be an important factor in their impaired host-defense. Twenty-nine patients in fulminant hepatic failure were studied on admission, and the mean fibronectin level in Grade 0-2 encephalopathy was 82 micrograms per ml (range = 0 to 150) and in Grade 3-4 encephalopathy 61 micrograms per ml (range = 5 to 158) as compared to normal controls (268 micrograms per ml, range = 178 to 380, n = 62). No fibronectin degradation products could be detected in fulminant hepatic failure plasma by sodium dodecyl sulfate-gel electrophoresis on a polyacrylamide gradient (5 to 15%) followed by immunoblotting onto nitrocellulose with detection using a rabbit antihuman fibronectin antiserum visualized with a peroxidase conjugate. The plasma levels of the marker proteolytic enzyme cathepsin D were significantly elevated in fulminant hepatic failure (120 +/- 31 mU per ml per hr) as compared to the normal controls (18 +/- 2.1 mU per ml per hr, n = 10, p less than 0.01). Cross-immunoelectrophoresis of fulminant hepatic failure plasma for fibronectin on agarose plates gave an additional slower migrating peak in 15 of the 29 patients, as well as that of fibronectin, which corresponded to the fibronectin complex reported by other workers in leukemia. An intermediate gel containing antihuman fibrinogen demonstrated fibrinogen to be one component of this complex. Binding of other substances to fibronectin will reduce its apparent biological activity and may be the result of their lack of clearance by the damaged liver.
暴发性肝衰竭患者体内调理素糖蛋白纤连蛋白的血浆水平降低，这可能是其宿主防御功能受损的一个重要因素。对29例暴发性肝衰竭患者入院时进行了研究，0 - 2级肝性脑病患者的纤连蛋白平均水平为每毫升82微克（范围 = 0至150），3 - 4级肝性脑病患者为每毫升61微克（范围 = 5至158），而正常对照组为每毫升268微克（范围 = 178至380，n = 62）。在聚丙烯酰胺梯度（5%至15%）的十二烷基硫酸钠 - 凝胶电泳后，将其免疫印迹到硝酸纤维素膜上，并用兔抗人纤连蛋白抗血清检测，再用过氧化物酶结合物显色，结果在暴发性肝衰竭血浆中未检测到纤连蛋白降解产物。与正常对照组（每毫升每小时18 ± 2.1毫单位，n = 10，p < 0.01）相比，暴发性肝衰竭患者体内标志物蛋白水解酶组织蛋白酶D的血浆水平显著升高（每毫升每小时120 ± 31毫单位）。在琼脂糖平板上对暴发性肝衰竭血浆中的纤连蛋白进行交叉免疫电泳，29例患者中有15例除了纤连蛋白峰外，还出现了一个迁移较慢的额外峰，这与其他研究人员在白血病中报道的纤连蛋白复合物相对应。含有抗人纤维蛋白原的中间凝胶显示纤维蛋白原是该复合物的一个成分。其他物质与纤连蛋白结合会降低其表观生物学活性，这可能是受损肝脏对它们清除不足的结果。
