Ferrini S, Moretta L, Pantaleo G, Moretta A
Int J Cancer. 1987 Jan 15;39(1):18-24. doi: 10.1002/ijc.2910390105.
Lymphokine-activated killer (LAK) activity was first analyzed on PBL populations fractionated on the basis of the expression of T11 or T3 antigen. LAK cell precursors were found to be present in both T11+ and T11- populations, but only in the T3- cell fraction. The generation of LAK activity in highly purified T3- populations of PBL was not accompanied by expression of T3 antigen during a 5-day culture period. LAK activity was next analyzed at the level of limiting dilution clonal microcultures. T11+T3- and T11+T3+ cells, cloned under optimal culture conditions, gave a frequency of proliferating cells of approximately 1 cell in 1.25 for T11+T3+ and 1 cell in 10 for T11+T3- cells. Clones were screened for their ability to lyse fresh ovarian carcinoma cells and K562 target cells. The majority of LAK clones were derived from the T11+T3- cells; moreover, most of the clones derived from these cells displayed LAK activity. Clones displaying LAK activity lysed a panel of fresh or cultured tumor target cells, but failed to lyse PHA-activated lymphoblasts. Surface marker analysis indicated that all the clones had maintained the original T11/T3 phenotype. Whereas 2 T3+ selected LAK clones expressed the T8+T4- phenotype, only 1 out of 9 T3- clones was T8+T4-, all the others lacking both T4 and T8 antigens.
首先在根据T11或T3抗原表达进行分离的外周血淋巴细胞（PBL）群体上分析淋巴因子激活的杀伤细胞（LAK）活性。发现LAK细胞前体存在于T11 +和T11 -群体中，但仅存在于T3 -细胞组分中。在5天的培养期内，PBL的高度纯化的T3 -群体中LAK活性的产生并未伴随着T3抗原的表达。接下来在有限稀释克隆微培养水平上分析LAK活性。在最佳培养条件下克隆的T11 + T3 -和T11 + T3 +细胞，T11 + T3 +细胞的增殖细胞频率约为1.25个细胞中有1个，T11 + T3 -细胞为10个细胞中有1个。筛选克隆裂解新鲜卵巢癌细胞和K562靶细胞的能力。大多数LAK克隆源自T11 + T3 -细胞；此外，源自这些细胞的大多数克隆显示出LAK活性。显示LAK活性的克隆裂解一组新鲜或培养的肿瘤靶细胞，但不能裂解PHA激活的淋巴母细胞。表面标志物分析表明所有克隆都维持了原始T11 / T3表型。虽然2个T3 +选择的LAK克隆表达T8 + T4 -表型，但9个T3 -克隆中只有1个是T8 + T4 -，其他所有克隆都缺乏T4和T8抗原。
