Watson R R, Lim T S
Int J Immunopharmacol. 1986;8(6):545-52. doi: 10.1016/0192-0561(86)90024-x.
The present studies were performed to determine the effects of severe protein deficiency and subsequent injection of thymosin fraction 5 (TF5) on T and B cell functions. BALB/c mice, 4 weeks old, were fed a normal protein (21%), a low protein (4%) or a protein free (0%) diet and then injected with TF5 or buffer (PBS). A significant increase was observed in the PHA (phytohemagglutinin) and LPS (lipopolysaccharide) induced mitogenesis with increasing age of the well-nourished, PBS injected animals. The severely protein malnourished mice, PBS injected and the well nourished mice, injected with TF5 had smaller increases in both B and T cell mitogenesis with increasing age. TF5 injection of the malnourished mice increased PHA and LPS mitogenesis nearly to the levels of the well-nourished mice. The protein malnourished mice consistently had higher serum corticosteroid levels than controls. No changes in serum corticosteroids were observed with TF5 injection of controls, but there was a significant decrease in the corticosteroid levels of the severely malnourished with TF5 injection. Cytoxicity assays of T cell function, antibody dependent cellular cytoxicity and cytoxicity to mouse thymona tumor cells, in mice fed moderately protein deficient diets showed suppression compared to controls fed 20% protein. TF5 injection partially and temporarily increased these functions in the malnourished mice.
进行本研究以确定严重蛋白质缺乏及随后注射胸腺素组分5（TF5）对T细胞和B细胞功能的影响。给4周龄的BALB/c小鼠喂食正常蛋白质（21%）、低蛋白质（4%）或无蛋白质（0%）饮食，然后注射TF5或缓冲液（PBS）。在注射PBS的营养良好的动物中，随着年龄增长，观察到PHA（植物血凝素）和LPS（脂多糖）诱导的有丝分裂显著增加。注射PBS的严重蛋白质营养不良小鼠和注射TF5的营养良好小鼠，随着年龄增长，B细胞和T细胞有丝分裂的增加幅度较小。给营养不良小鼠注射TF5可使PHA和LPS有丝分裂几乎增加到营养良好小鼠的水平。蛋白质营养不良小鼠的血清皮质类固醇水平始终高于对照组。给对照组注射TF5后，血清皮质类固醇未见变化，但给严重营养不良小鼠注射TF5后，皮质类固醇水平显著降低。与喂食20%蛋白质的对照组相比，喂食中度蛋白质缺乏饮食的小鼠中T细胞功能的细胞毒性测定、抗体依赖性细胞毒性以及对小鼠胸腺瘤肿瘤细胞的细胞毒性均表现出抑制作用。给营养不良小鼠注射TF5可部分且暂时增加这些功能。
