College of Pharmaceutical Science, Zhejiang University of Technology, Hangzhou 310014, China.
College of Materials Science and Engineering, Zhejiang University of Technology, Hangzhou 310014, China.
Molecules. 2019 Apr 3;24(7):1312. doi: 10.3390/molecules24071312.
In this study, a green ionic-liquid based vortex-synchronized matrix solid-phase dispersion (VS-MSPD) combined with high performance liquid chromatography (HPLC) method was developed as a quantitative determination method for four anthraquinones in Cassiae Semen. Two conventional adsorbents, C and silica gel were investigated. The strategy included two steps: Extraction and determination. Wasted crab shells were used as an alternative adsorbent and ionic liquid was used as an alternative solvent in the first step. Factors affecting extraction efficiency were optimized: A sample/adsorbent ratio of 2:1, a grinding time of 3 min, a vortex time of 3 min, and ionic liquid ([Domim]HSO₄, 250 mM) was used as eluent in the VS-MSPD procedure. As a result, the established method provided satisfactory linearity (R > 0.999), good accuracy and high reproducibility (RSD < 4.60%), and it exhibited the advantages of smaller sample amounts, shorter extraction time, less volume of elution solvent, and was much more environmental-friendly when compared with other conventional methods.
在本研究中，开发了一种基于绿色离子液体的涡旋同步基质固相分散（VS-MSPD）结合高效液相色谱（HPLC）方法，作为定量测定决明子中四种蒽醌类化合物的方法。研究了两种常规吸附剂 C 和硅胶。该策略包括两个步骤：提取和测定。第一步中，将废弃的蟹壳用作替代吸附剂，离子液体用作替代溶剂。优化了影响萃取效率的因素：样品/吸附剂比为 2:1，研磨时间为 3 分钟，涡旋时间为 3 分钟，VS-MSPD 过程中使用离子液体 ([Domim]HSO₄，250 mM) 作为洗脱液。结果，所建立的方法提供了令人满意的线性（R > 0.999）、良好的准确性和高重现性（RSD < 4.60%），与其他常规方法相比，它具有样品量小、提取时间短、洗脱溶剂体积少、更环保的优点。
