Tianjin Key Laboratory of Phytochemistry and Pharmaceutical Analysis, Tianjin University of Traditional Chinese Medicine, Tianjin, China.
Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin, China.
J Food Biochem. 2019 Apr;43(4):e12807. doi: 10.1111/jfbc.12807. Epub 2019 Feb 14.
A simple miniaturized matrix solid-phase dispersion extraction was developed to analyze multiple antioxidant ingredients (neochlorogenic acid, chlorogenic acid, cryptochlorogenic acid, rutin, isoquercitrin, and astragalin) in Mori Fructus by ultra performance liquid chromatography coupled with an ultraviolet detector. 1-ethyl-3-methylimidazolium bromide was employed in the elution process as eco-friendly elution solvent. The effects of some factors on the extraction efficiency of antioxidants in Mori Fructus were optimized in detail, such as the type of adsorbent, the type of elution solvent, the concentration and volume of elution solvent, sample to adsorbent ratio, and the grinding time. The intra-day and inter-day precisions (RSD) were both below 5.0% and the recoveries of all analytes ranged from 93.5% to 98.3%. Compared with the traditional method, it was efficient, simple and environment-friendly for extracting the analytes by ionic liquid assisted trace β-CD matrix solid-phase dispersion extraction method, due to shorter extraction time, less reagent and less sample consumption. The developed method was successfully used for extracting and determining the active compounds in Mori Fructus sample. PRACTICAL APPLICATIONS: The antioxidant ingredients play important roles in Mori Fructus because of their main pharmacological activities. Nowadays, a few analytical methods could be applied for extracting and analyzing these target compounds. But these methods not only required much extracting and analyzing time, but also need a great deal of organic reagent, which were not environmentally friendly. Thus, a green and simple miniaturized matrix solid phase dispersion extraction method was proposed for the analysis of these antioxidants in Mori Fructus, which was efficient to be employed for evaluating the quality of Traditional Chinese Medicine.
一种简单的微型基质固相分散萃取方法被开发出来，用于通过超高效液相色谱法与紫外检测器分析桑白皮中的多种抗氧化成分（新绿原酸、绿原酸、隐绿原酸、芦丁、异槲皮苷和黄芪苷）。在洗脱过程中使用 1-乙基-3-甲基咪唑溴盐作为环保洗脱溶剂。详细优化了一些因素对桑白皮中抗氧化剂提取效率的影响，例如吸附剂的类型、洗脱溶剂的类型、洗脱溶剂的浓度和体积、样品与吸附剂的比例以及研磨时间。日内和日间精密度（RSD）均低于 5.0%，所有分析物的回收率均在 93.5%至 98.3%之间。与传统方法相比，离子液体辅助痕量β-CD 基质固相分散萃取法提取分析物的效率高、简单、环保，因为它的提取时间更短、试剂和样品用量更少。该方法成功用于提取和测定桑白皮样品中的活性化合物。实际应用：抗氧化成分在桑白皮中发挥着重要作用，因为它们具有主要的药理活性。目前，只有少数分析方法可用于提取和分析这些目标化合物。但是，这些方法不仅需要大量的提取和分析时间，而且还需要大量的有机溶剂，这不环保。因此，提出了一种绿色、简单的微型基质固相分散萃取方法，用于分析桑白皮中的这些抗氧化成分，该方法可用于评估中药的质量。
