Determination of antioxidant ingredients in Mori Fructus employing ionic liquid-assisted miniaturized matrix solid-phase dispersion extraction via ultra-performance liquid chromatography.

A simple miniaturized matrix solid-phase dispersion extraction was developed to analyze multiple antioxidant ingredients (neochlorogenic acid, chlorogenic acid, cryptochlorogenic acid, rutin, isoquercitrin, and astragalin) in Mori Fructus by ultra performance liquid chromatography coupled with an ultraviolet detector. 1-ethyl-3-methylimidazolium bromide was employed in the elution process as eco-friendly elution solvent. The effects of some factors on the extraction efficiency of antioxidants in Mori Fructus were optimized in detail, such as the type of adsorbent, the type of elution solvent, the concentration and volume of elution solvent, sample to adsorbent ratio, and the grinding time. The intra-day and inter-day precisions (RSD) were both below 5.0% and the recoveries of all analytes ranged from 93.5% to 98.3%. Compared with the traditional method, it was efficient, simple and environment-friendly for extracting the analytes by ionic liquid assisted trace β-CD matrix solid-phase dispersion extraction method, due to shorter extraction time, less reagent and less sample consumption. The developed method was successfully used for extracting and determining the active compounds in Mori Fructus sample. PRACTICAL APPLICATIONS: The antioxidant ingredients play important roles in Mori Fructus because of their main pharmacological activities. Nowadays, a few analytical methods could be applied for extracting and analyzing these target compounds. But these methods not only required much extracting and analyzing time, but also need a great deal of organic reagent, which were not environmentally friendly. Thus, a green and simple miniaturized matrix solid phase dispersion extraction method was proposed for the analysis of these antioxidants in Mori Fructus, which was efficient to be employed for evaluating the quality of Traditional Chinese Medicine.