在成年斑马鱼中，[某种物质或机制]的抑制作用调节冷冻损伤后的心脏再生。 （注：原文中“Inhibition of ”后面缺少具体内容，这里只能按大概意思翻译）
Inhibition of Regulates Cardiac Regeneration after Cryoinjury in Adult Zebrafish.
作者信息
Narumanchi Suneeta, Kalervo Karri, Perttunen Sanni, Wang Hong, Immonen Katariina, Kosonen Riikka, Laine Mika, Ruskoaho Heikki, Tikkanen Ilkka, Lakkisto Päivi, Paavola Jere
机构信息
Unit of Cardiovascular Research, Minerva Institute for Medical Research, Biomedicum Helsinki, 00290 Helsinki, Finland.
Department of Surgery, South Karelia Central Hospital, 53130 Lappeenranta, Finland.
出版信息
J Cardiovasc Dev Dis. 2019 Apr 4;6(2):16. doi: 10.3390/jcdd6020016.
The family of micro-RNAs (miRNAs) is expressed during embryonic development and plays an important role in cell differentiation. We have investigated the role of in heart regeneration after injury in adult zebrafish. antagomir or scramble injections were given at one day after cryoinjury (1 dpi). Tissue samples were collected at 7 dpi, 14 dpi and 28 dpi and cardiac function was assessed before cryoinjury, 1 dpi, 7 dpi, 14 dpi and 28 dpi. Inhibition of increased the rate of fibrinolysis, increased the number of proliferating cell nuclear antigen (PCNA) positive cardiomyocytes at 7 dpi and increased the expression of the epicardial marker at 7 dpi. Additionally, cardiac function measured with echocardiography recovered slightly more rapidly after inhibition of . These results reveal a beneficial role of inhibition in adult zebrafish heart regeneration.
微小RNA（miRNA）家族在胚胎发育过程中表达，并在细胞分化中起重要作用。我们研究了其在成年斑马鱼心脏损伤后再生中的作用。在冷冻损伤后一天（1 dpi）注射抗miR或乱序序列。在7 dpi、14 dpi和28 dpi收集组织样本，并在冷冻损伤前、1 dpi、7 dpi、14 dpi和28 dpi评估心脏功能。抑制该miRNA增加了纤维蛋白溶解速率，在7 dpi时增加了增殖细胞核抗原（PCNA）阳性心肌细胞的数量，并在7 dpi时增加了心外膜标志物的表达。此外，在抑制该miRNA后，用超声心动图测量的心脏功能恢复得稍快一些。这些结果揭示了抑制该miRNA在成年斑马鱼心脏再生中的有益作用。
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