Takle G B, Lackie A M
J Cell Sci. 1986 Sep;85:85-94. doi: 10.1242/jcs.85.1.85.
Time-lapse microphotography was used to film the locomotory behaviour of cockroach haemocytes in vitro, and the cell tracks were analysed for speed and persistence; the percentage mobilization and the diffusion rate of the population were calculated. Haemocytes are either fast locomotor or spread moving cells, or non-motile spread or rounded cells; the first three types are plasmatocytes and their behaviour is interchangeable. Approximately 20% of the cells are motile under control conditions and there is no correlation between orthokinesis and klinokinesis. If activated haemocyte lysate supernatant (HLS), a source of components of the prophenoloxidase enzyme sequence, is added to the cell monolayer, up to 80% of the cells switch to fast locomotor behaviour, rounding up and moving faster and for longer in straight lines. Neither heat-inactivated HLS nor zymosan supernatant, used to activate HLS, had any effect. If the chemokinins present in activated HLS are also released in vivo on haemocyte activation or during cuticular wounding, then they and the induced changes in haemocyte adhesion could contribute to haemocyte recruitment to sites of infection.
采用延时显微摄影技术对蟑螂血细胞的体外运动行为进行拍摄，并对细胞轨迹的速度和持续性进行分析；计算细胞群体的动员百分比和扩散速率。血细胞分为快速运动或铺展移动的细胞，或不运动的铺展或圆形细胞；前三种类型是浆细胞，它们的行为是可以互换的。在对照条件下，约20%的细胞具有运动能力，直线运动速度和转向运动速度之间没有相关性。如果将作为酚氧化酶原酶序列成分来源的活化血细胞裂解物上清液（HLS）添加到细胞单层中，高达80%的细胞会转变为快速运动行为，变圆并在直线上更快、更持久地移动。热灭活的HLS或用于激活HLS的酵母聚糖上清液均无任何作用。如果活化HLS中存在的趋化激动素在体内血细胞活化或表皮受伤时也会释放，那么它们以及诱导的血细胞黏附变化可能有助于将血细胞募集到感染部位。
