Xu Zuqiang, Wu Hanyan, Tang Yang, Xu Wei, Zhai Yanbing
School of Life Science, Beijing Institute of Technology, Beijing, 100081, China.
Beijing Institute for Drug Control, Beijing, 102206, China.
J Mass Spectrom. 2019 Jul;54(7):583-591. doi: 10.1002/jms.4361.
A better understanding of nanoelectrospray ionization (nano-ESI) would be beneficial in further improving the performances of nano-ESI. In this work, the pulsed high-voltage (HV) nano-ESI has been electrically modeled and then systematically characterized by both voltage-current and mass spectrometry measurements. First, the equivalent resistance of a nano-ESI source changes with respect to both emitter tip diameter and the HV applied. Increased voltage could improve both spray current and ionization efficiency of the pulsed HV nano-ESI. Compared with conventional DC HV method, a pulsed HV has less heating effect on the capillary tip and thus allowing the application of a much higher voltage onto a nano-ESI source. As a result, a pulsed HV nano-ESI could further boost the ionization efficiency of nano-ESI by employing even higher voltages than conventional DC nano-ESI sources.
更好地理解纳米电喷雾电离（nano-ESI）将有助于进一步提高nano-ESI的性能。在这项工作中，对脉冲高压（HV）nano-ESI进行了电学建模，然后通过电压-电流和质谱测量进行了系统表征。首先，nano-ESI源的等效电阻随发射极尖端直径和所施加的HV而变化。增加电压可以提高脉冲HV nano-ESI的喷雾电流和电离效率。与传统的直流HV方法相比，脉冲HV对毛细管尖端的加热效应较小，因此可以在nano-ESI源上施加更高的电压。结果，脉冲HV nano-ESI通过采用比传统直流nano-ESI源更高的电压，可以进一步提高nano-ESI的电离效率。
