Ge Ya-Zhe, Ma Qing-Yun, Kong Fan-Dong, Xie Qing-Yi, An Chang-Liang, Yu Zhi-Fang, Zhao You-Xing
College of Food Science and Technology, Nanjing Agricultural University Nanjing 210095, China Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences Haikou 571101, China.
Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences Haikou 571101, China.
Zhongguo Zhong Yao Za Zhi. 2019 Feb;44(3):489-494. doi: 10.19540/j.cnki.cjcmm.20181121.004.
Chemical constituents were isolated from the fruiting bodies of Ganoderma australe by various column chromatographic techniques and HPLC method, and their chemical structures were identified through the combined analysis of physicochemical properties and spectral data. Meanwhile, their α-glucosidase inhibitory activity and anti-oxidative ability were evaluated. Seven compounds were isolated from G. australe and were identified as 6-methoxyl-cyclo-(Phe-Ile)(1), applanoxidic acid A methyl ester(2), ergosta-7,22 E-dien-3β-ol(3), cinnamic acid(4), 5α,8α-epidioxy-(20S,22E,24R)-ergosta-6,22-diene-3β-ol(5), 1-(3, 4-dihydroxyphenyl) ethanone(6), salicylic acid(7) and benzoic acid(8). Among the compounds, compound 1 was a new cyclic dipeptide. Compound 2 was a new lanosta natural product, and compounds 4, 6, 7 and 8 were obtained from G. australe for the first time. Moreover, compounds 4 and 8 exhibited α-glucosidase inhibitory activity with inhibition rates of 36.8% and 34.7%, and compounds 4, 7 and 8 had a certain activity in DPPH free radical scavenging activity with IC_(50) values of 0.168, 0.458 and 0.170 g·L~(-1), respectively. The DPPH radical scavenging rate of compound 1 was 41.1%.
采用多种柱色谱技术和高效液相色谱法从南方灵芝子实体中分离化学成分，并通过理化性质和光谱数据的综合分析鉴定其化学结构。同时，评价了它们的α-葡萄糖苷酶抑制活性和抗氧化能力。从南方灵芝中分离得到7个化合物，分别鉴定为6-甲氧基-环-(苯丙氨酸-异亮氨酸)(1)、扁平氧化酸A甲酯(2)、麦角甾-7,22E-二烯-3β-醇(3)、肉桂酸(4)、5α,8α-环氧-(20S,22E,24R)-麦角甾-6,22-二烯-3β-醇(5)、1-(3,4-二羟基苯基)乙酮(6)、水杨酸(7)和苯甲酸(8)。其中，化合物1为新的环二肽。化合物2为新的羊毛甾烷天然产物，化合物4、6、7和8为首次从南方灵芝中获得。此外，化合物4和8表现出α-葡萄糖苷酶抑制活性，抑制率分别为36.8%和34.7%，化合物4、7和8在DPPH自由基清除活性方面具有一定活性，IC_(50)值分别为0.168、0.458和0.170 g·L~(-1)。化合物1的DPPH自由基清除率为41.1%。
