[三种中药辛辣挥发油对苦参生物碱经皮吸收的影响]

[Effects of pungent essential oil from three Chinese herbs on percutaneous absorption of alkaloids from Sophorae Flavescentis Radix].

作者信息

Wang Ling, Zhang Ting, Qian Da-Wei, Zhu Zhen-Hua, Wang Chun-Xue, Weng Ze-Bin, Zhang Huang-Qin, Guo Sheng, Su Shu-Lan, Duan Jin-Ao

机构信息

Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization/Jiangsu Key Laboratory for High Technology Research of Traditional Chinese Medicine Formulae,Nanjing University of Chinese Medicine Nanjing 210023,China National and Local Collaborative Engineering Center of Chinese Medicinal Resources Industrialization and Formulae Innovative Medicine,Nanjing University of Chinese Medicine Nanjing 210023,China.

出版信息

Zhongguo Zhong Yao Za Zhi. 2019 Jan;44(2):308-313. doi: 10.19540/j.cnki.cjcmm.20181108.001.

DOI:10.19540/j.cnki.cjcmm.20181108.001

PMID:30989950

Abstract

To investigate the effects of essential oil from three kinds of pungent herbs,namely Menthae Haplocalycis Herba,Atractylodis Rhizoma and Cnidii Fructus,on the transdermal absorption in vitro of alkaloids from Sophorae Flavescentis Radix. The modified vertical Franz diffusion cell was used to conduct a transdermal experiment in vitro with the isolated abdominal skin of the SD rats as the transdermal absorption barrier. The effects of such three kinds of pungent essential oil on percutaneous absorption of alkaloids from Sophorae Flavescentis Radix were investigated by determining the content of 6 alkaloids( oxymatrine,oxysophocarpine,N-methylcytisine,sophoridine,matrine,and sophocarpidine) in the transdermal acceptor with ultra-performance liquid chromatography-triple quadruple mass spectrometry( UPLC-TQ-MS) technique simultaneously. With enhancement ratio( ER) as the index,their effects on promoting penetration was as follows: 1% Atractylodis Rhizoma oil > 1% Cnidii Fructus oil > 3% Azone ≈ 3% Atractylodis Rhizoma oil > 5%Atractylodis Rhizoma oil > 3% Cnidii Fructus oil ≈ 5% Cnidii Fructus oil > 3% Menthae Haplocalycis Herba oil > 5% Menthae Haplocalycis Herba oil > 1% Menthae Haplocalycis Herba oil > Blank. The results showed that these three kinds of pungent essential oil could be used as enhancers for alkaloids of Sophorae Flavescentis Radix,providing scientific guidance for improving percutaneous absorption of alkaloids from Sophorae Flavescentis Radix.

摘要

考察三种辛味药材薄荷、苍术、蛇床子挥发油对苦参生物碱体外透皮吸收的影响。采用改良的垂直式Franz扩散池，以SD大鼠离体腹部皮肤为透皮吸收屏障进行体外透皮实验。采用超高效液相色谱-三重四极杆质谱联用（UPLC-TQ-MS）技术，通过测定透皮接受液中6种生物碱（氧化苦参碱、氧化槐果碱、N-甲基野靛碱、槐定碱、苦参碱、槐果碱）的含量，考察三种辛味挥发油对苦参生物碱经皮吸收的影响。以促渗率（ER）为指标，其促渗作用大小依次为：1%苍术油＞1%蛇床子油＞3%氮酮≈3%苍术油＞5%苍术油＞3%蛇床子油≈5%蛇床子油＞3%薄荷油＞5%薄荷油＞1%薄荷油＞空白。结果表明，三种辛味挥发油可作为苦参生物碱的促渗剂，为提高苦参生物碱经皮吸收提供科学指导。