Lin Man, Wang Yu-Rong, Zhai Xin-Fang, Xing Shao-Fang, Piao Xiang-Lan
School of Pharmacy,Minzu University of China Beijing 100081,China.
Zhongguo Zhong Yao Za Zhi. 2019 Mar;44(6):1193-1200. doi: 10.19540/j.cnki.cjcmm.20181226.019.
Four flavonoids were isolated from Gynostemma pentaphyllum by chromatography methods and their structures were identified by MS and NMR spectra data as quercetin-3-O-( 2″,6″-di-α-L-rhamnosyl)-β-D-galactopyranoside( 1),quercetin-3-O-( 2″,6″-di-α-L-rhamnosyl)-β-D-glucopyranoside( 2),quercetin-3-O-( 2″-α-L-rhamnosyl)-β-D-galactopyranoside( 3),and quercetin-3-O-( 2″-α-L-rhamnosyl)-β-D-glucopyranoside( 4). Among them,compounds 1-3 were obtained from the Cucurbitaceae family for the first time.The four flavonoids showed potent antioxidant effects against the DPPH,·OH and ■radicals in vitro,especially for DPPH radical scavenging activity with the IC50 values of 71. 4,29. 5,48. 3 and 79. 2 μmol·L~(-1),respectively. Moreover,the four flavonoids displayed strong cytoprotection against AAPH-induced oxidative damage in LLC-PK1 cells by suppressing the increase of malondialdehyde( MDA) and the decrease of the superoxide dismutase( SOD) and glutathione( GSH). Since further research is needed to prove its efficacy in vivo and clinical trial,the study may provide four potential antioxidants from G. pentaphyllum.
通过色谱法从绞股蓝中分离出四种黄酮类化合物，并通过质谱和核磁共振光谱数据将其结构鉴定为槲皮素-3-O-(2″,6″-二-α-L-鼠李糖基)-β-D-吡喃半乳糖苷(1)、槲皮素-3-O-(2″,6″-二-α-L-鼠李糖基)-β-D-吡喃葡萄糖苷(2)、槲皮素-3-O-(2″-α-L-鼠李糖基)-β-D-吡喃半乳糖苷(3)和槲皮素-3-O-(2″-α-L-鼠李糖基)-β-D-吡喃葡萄糖苷(4)。其中，化合物1-3首次从葫芦科植物中获得。这四种黄酮类化合物在体外对DPPH、·OH和■自由基显示出强大的抗氧化作用，尤其是对DPPH自由基的清除活性，IC50值分别为71.4、29.5、48.3和79.2 μmol·L~(-1)。此外，这四种黄酮类化合物通过抑制丙二醛(MDA)的增加以及超氧化物歧化酶(SOD)和谷胱甘肽(GSH)的减少，对AAPH诱导的LLC-PK1细胞氧化损伤表现出强大的细胞保护作用。由于需要进一步研究来证明其体内疗效和进行临床试验，该研究可能从绞股蓝中提供四种潜在的抗氧化剂。
