Suemura M, Urban J F, Ishizaka K
J Immunol. 1978 Dec;121(6):2413-21.
Mesenteric lymph node cells from normal rats and rats infected with Nippostrongylus brasiliensis (Nb) were cultured with pokeweed mitogen (PWM) or Nb antigen, and the development of IgM-, IgG2a-, or IgE-containing cells was assessed by immunofluorescence. Normal lymph node cells stimulated with PWM developed into both IgM- and IgE-containing cells, whereas similar stimulation of cells from Nb-infected rats resulted in the development of IgM-, IgG2a-, and IgE-containing cells. The in vitro plasma cell response to PWM was dependent on the presence of T lymphocytes. Lymph node cells from Nb-infected rats responsed to Nb antigen and developed into plasma cells of IgM, IgG, and IgE classes. The response was antigen specific and required antigen-primed T cells. Depletion of IgE-bearing cells or IgM-bearing cells before stimulation with either PWM or Nb antigen diminished the level of IgE forming cell development, suggesting that IgE-IgM double bearing cells are precursors of IgE-forming cells. The distribution of the three isotypes among the If-forming cells that developed in response to PWM was influenced by the source of both B and T cells. When B cells from Nb-infected rats were employed as a source of precursors, T cells from infected animals were more effective than normal T cells for the development of IgE-forming cells, whereas the latter cells were more effective for the development of IgG2a-forming cells than T cells from infected animals.
将正常大鼠和感染巴西日圆线虫（Nb）的大鼠的肠系膜淋巴结细胞与商陆有丝分裂原（PWM）或Nb抗原一起培养，并用免疫荧光法评估含IgM、IgG2a或IgE细胞的发育情况。用PWM刺激的正常淋巴结细胞发育为含IgM和IgE的细胞，而对感染Nb的大鼠的细胞进行类似刺激则导致含IgM、IgG2a和IgE的细胞发育。体外浆细胞对PWM的反应取决于T淋巴细胞的存在。感染Nb的大鼠的淋巴结细胞对Nb抗原产生反应并发育为IgM、IgG和IgE类别的浆细胞。该反应具有抗原特异性，需要抗原致敏的T细胞。在用PWM或Nb抗原刺激之前，去除含IgE的细胞或含IgM的细胞会降低IgE形成细胞的发育水平，这表明IgE-IgM双阳性细胞是IgE形成细胞的前体。在对PWM产生反应而发育的形成If的细胞中，三种同种型的分布受B细胞和T细胞来源的影响。当使用感染Nb的大鼠的B细胞作为前体细胞来源时，感染动物的T细胞比正常T细胞对IgE形成细胞的发育更有效，而后者细胞对IgG2a形成细胞的发育比感染动物的T细胞更有效。
