Matsuda Kazunori, Kitahara Tadashi, Ito Taeko, Fukushima Munehisa, Fukuda Junya, Sato Go, Kitamura Yoshiaki, Abe Koji, Uno Atsuhiko, Tomita Koichi, Sakata-Haga Hiromi, Fukui Yoshihiro, Takeda Noriaki
a Department of Otolaryngology , Institute of Biomedical Sciences, Tokushima University Graduate School , Tokushima , Japan.
b Department of Otolaryngology , Nara Medical University , Kashihara , Japan.
Acta Otolaryngol. 2019 Jun;139(6):505-510. doi: 10.1080/00016489.2019.1599140. Epub 2019 Apr 16.
Unilateral labyrinthectomy (UL) causes the disappearance of ipsilateral medial vestibular nuclear (ipsi-MVe) activity and induces spontaneous nystagmus (SN), which disappears during the initial process of vestibular compensation (VC). Ipsi-MVe-activity restores in the late process of VC.
We evaluated the late process of VC after UL in rats and examined the effects of thioperamide (H3 antagonist) on VC.
MK801 (NMDA antagonist)-induced Fos-like immunoreactive (-LIR) neurons in contra-MVe, which had been suppressed by NMDA-mediated cerebellar inhibition in UL rats was used as an index.
The number of MK801-induced Fos-LIR neurons in contra-MVe gradually decreased to the same level as that of sham-operated rats 14 days after UL. Thioperamide moved the disappearance of the MK801-induced Fos-LIR neurons 2 days earlier. The number of MK801-induced Fos-LIR neurons in thioperamide-treated rats was significantly decreased, compared with that of vehicle rats on days 7 and 12 after UL. But, thioperamide did not influence the decline of SN frequency in UL rats.
These findings suggested that the number of MK801-induced Fos-LIR neurons in contra-MVe was decreased in concordance with the restoration of ipsi-MVe-activity during the late process of VC after UL and that thioperamide accelerated the late, but not the initial process of VC.
单侧迷路切除术（UL）导致同侧内侧前庭核（ipsi-MVe）活动消失，并诱发自发性眼球震颤（SN），在前庭代偿（VC）的初始过程中该眼球震颤消失。Ipsi-MVe活动在VC后期恢复。
我们评估了大鼠UL后VC的后期过程，并研究了硫代哌酰胺（H3拮抗剂）对VC的影响。
以MK801（NMDA拮抗剂）诱导的对侧MVe中Fos样免疫反应性（-LIR）神经元为指标，该神经元在UL大鼠中被NMDA介导的小脑抑制所抑制。
UL后14天，MK801诱导的对侧MVe中Fos-LIR神经元数量逐渐减少至与假手术大鼠相同水平。硫代哌酰胺使MK801诱导的Fos-LIR神经元消失提前2天。与UL后第7天和第12天的溶剂对照组大鼠相比，硫代哌酰胺处理组大鼠中MK801诱导的Fos-LIR神经元数量显著减少。但是，硫代哌酰胺不影响UL大鼠中SN频率的下降。
这些发现表明，在UL后VC的后期过程中，MK801诱导的对侧MVe中Fos-LIR神经元数量的减少与ipsi-MVe活动的恢复一致，并且硫代哌酰胺加速了VC的后期过程，但未加速其初始过程。
