Shah Sujit, Thapa Bir Bhadur, Chand Krishna, Pradhan Shreeti, Singh Anjana, Varma Ajit, Sen Thakuri Laxmi, Joshi Puspraj, Pant Bijaya
a Central Department of Botany , Tribhuvan University , Kathmandu , Nepal.
b Central Department of Microbiology , Tribhuvan University , Kathmandu , Nepal.
Plant Signal Behav. 2019;14(6):1596716. doi: 10.1080/15592324.2019.1596716. Epub 2019 Apr 16.
Cymbidium aloifolium is known for its ornamental and medicinal values. It has been listed as threatened orchid species. In this study, in vitro propagated C. aloifolium plantlets were interacted with the Piriformospora indica. The growth assay was performed for 45 days; the plant growth pattern such as number and length of roots and shoots were measured. Microscopic study of the root section stained by trypan blue was done to detect the peloton formation. The methanol extracts of the fungal colonized plant as well as uncolonized (control) plant were prepared and various metabolites were identified by gas chromatography mass spectroscopy. Acclimatization was done in a substrate composition of coco peat: gravel: charcoal in ratio 2:2:1. P. indica-colonized plantlet showed the highest growth with the formation of clamdospore in the root section. The growth regulator such as auxin, ascorbic acid, andrographolide, hexadecanoic acid, and DL-proline were identified. After three months of field transfer, plantlet colonized by P. indica survived and remained healthy as compared to uncolonized control plantlet.
竹叶兰因其观赏价值和药用价值而闻名。它已被列为受威胁的兰花物种。在本研究中，对离体繁殖的竹叶兰组培苗与印度梨形孢进行了相互作用。进行了45天的生长测定；测量了植株的生长模式，如根和芽的数量及长度。对经台盼蓝染色的根段进行显微镜研究以检测丛枝的形成。制备了真菌定殖植株以及未定殖（对照）植株的甲醇提取物，并通过气相色谱-质谱法鉴定了各种代谢物。在椰糠：砾石：木炭比例为2:2:1的基质组合物中进行炼苗。印度梨形孢定殖的组培苗生长最好，根段中形成了厚垣孢子。鉴定出了生长素、抗坏血酸、穿心莲内酯、十六烷酸和DL-脯氨酸等生长调节剂。与未定殖的对照组培苗相比，经印度梨形孢定殖的组培苗在移栽到田间三个月后存活且保持健康。
