Shah Sujit, Paudel Mukti Ram, Thapa Bir Bahadur, Sharma Harshita, Kashyap Arun Kumar, Rekadwad Bhagwan Narayan, Sharma Rohit, Sharma Jyotsna, Pant Bijaya
Central Department of Botany, Tribhuvan University, Kirtipur, Kathmandu, Nepal.
Department of Plant Science and Soil Science, Texas Tech University, Lubbock, TX, USA.
Commun Integr Biol. 2024 Dec 17;18(1):2439798. doi: 10.1080/19420889.2024.2439798. eCollection 2025.
We isolated endophytic strains from the healthy roots, stems, and leaves of to investigate their plant growth-promoting activities in vitro. Subsequently, Indole acetic acid (IAA) was quantified and the gene (responsible for IAA synthesis in fungi) was amplified and sequenced. Finally, a germination assay was performed with seeds of and a plant growth assay with protocorms of to test their plant growth-promoting activities. Five isolates (CDS11, PDL1, PDL3, PDR6, PDR7) were identified in this study. The highest amount (60µgml) of indole acetic acid was recorded in the PDR7 extract, whereas it was not detected in PDR6 and CDS11. The fungal extracts of isolates PDR6 and PDR7 were highly effective for seed germination by approximately 80% and 90% (respectively) of the host plant. The fungal extract of PDR7 showed a high IAA content and promoted in vitro seed germination of the host () and protocorm development of the non-host (). In contrast, IAA content in the fungal extract of PDR6 remained undetected but was effective in both seed germination and protocorm development. Our results demonstrated the potential beneficial application of endophytic in orchid mass propagation.
我们从健康的根、茎和叶中分离内生菌株，以研究它们在体外促进植物生长的活性。随后，对吲哚乙酸（IAA）进行定量，并扩增和测序负责真菌中IAA合成的基因。最后，用种子进行发芽试验，并用原球茎进行植物生长试验，以测试它们促进植物生长的活性。本研究鉴定出5株分离株（CDS11、PDL1、PDL3、PDR6、PDR7）。在PDR7提取物中吲哚乙酸含量最高（60µg/ml），而在PDR6和CDS11中未检测到。分离株PDR6和PDR7的真菌提取物对宿主植物种子发芽的效果非常显著，分别约为80%和90%。PDR7的真菌提取物显示出高IAA含量，并促进了宿主（）的体外种子发芽和非宿主（）的原球茎发育。相比之下，PDR6的真菌提取物中IAA含量未检测到，但在种子发芽和原球茎发育方面均有效。我们的结果证明了内生菌在兰花大规模繁殖中的潜在有益应用。
