Wang Shuo, Wang Kai, Deng Zifeng, Jiang Zemin, Wang Dai, Yao Youliang, Guo Dongbei, Kong Xiangyu, Guan Zhiyu, Zhang Yongxing
Department of Thoracic Surgery, The Second Hospital of Tianjin Medical University, Tianjin, China.
State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, School of Public Health, Xiamen University, Xiamen Fujian, China.
Asia Pac J Clin Oncol. 2019 Oct;15(5):e162-e166. doi: 10.1111/ajco.13146. Epub 2019 Apr 16.
This study aimed to investigate the correlation between the expression of A-kinase anchor protein95 (AKAP95), p-retinoblastoma (phosphorylated Rb, p-Rb), cyclin D2, cyclin D3 and cyclin E2 in esophageal cancer tissues and clinicopathological indexes.
The protein expression levels of AKAP95, p-Rb, cyclin D2/3 and cyclin E2 in 40 esophageal cancer tissues were detected using immunohistochemistry, and the correlation between them was analyzed.
The percentage of p-Rb (Ser780)-, cyclin D2-, cyclin D3- and cyclin E2-positive samples was 62.50%, 70.00%, 67.50% and 60.00%, respectively. Also, the positive expression did not correlate with the histological type, histological differentiation or lymph node metastasis. The expression of AKAP95 and p-Rb (Ser780), p-Rb (Ser780) and cyclin D2 and p-Rb (Ser780) and cyclin D3 in esophageal cancer tissues was found to be correlated (P < 0.05).
The expression of AKAP95 and p-Rb (Ser780), p-Rb(Ser780) and cyclin D2, and p-Rb (Ser780) and cyclin D3 in esophageal cancer tissue was correlated, suggesting that these proteins might play a synergistic role in cell-cycle progression. Cyclin D2/D3 and p-Rb (Ser780) were correlated whereas cyclin E2 and p-Rb (Ser780) were not, suggesting that p-Rb (Ser780) might be highly expressed and the Ser780 site of Rb protein might be phosphorylated in the early stage of the G1 phase. Ser780 was the site in the primary phosphorylation stage of several phosphorylation sites during stepwise phosphorylation (from primary to high phosphorylation).
本研究旨在探讨食管癌组织中A激酶锚定蛋白95（AKAP95）、磷酸化视网膜母细胞瘤蛋白（p-Rb）、细胞周期蛋白D2、细胞周期蛋白D3和细胞周期蛋白E2的表达与临床病理指标之间的相关性。
采用免疫组织化学法检测40例食管癌组织中AKAP95、p-Rb、细胞周期蛋白D2/3和细胞周期蛋白E2的蛋白表达水平，并分析它们之间的相关性。
p-Rb（Ser780）、细胞周期蛋白D2、细胞周期蛋白D3和细胞周期蛋白E2阳性样本的百分比分别为62.50%、70.00%、67.50%和60.00%。此外，阳性表达与组织学类型、组织学分化或淋巴结转移无关。发现食管癌组织中AKAP95与p-Rb（Ser780）、p-Rb（Ser780）与细胞周期蛋白D2以及p-Rb（Ser780）与细胞周期蛋白D3的表达具有相关性（P<0.05）。
食管癌组织中AKAP95与p-Rb（Ser780）、p-Rb（Ser780）与细胞周期蛋白D2以及p-Rb（Ser780）与细胞周期蛋白D3的表达具有相关性，提示这些蛋白可能在细胞周期进程中发挥协同作用。细胞周期蛋白D2/D3与p-Rb（Ser780）相关，而细胞周期蛋白E2与p-Rb（Ser780）不相关，提示p-Rb（Ser780）可能高表达，且Rb蛋白的Ser780位点可能在G1期早期被磷酸化。Ser780是逐步磷酸化（从初级磷酸化到高级磷酸化）过程中几个磷酸化位点的初级磷酸化阶段的位点。
