Conceição M J, Coura J R
Mem Inst Oswaldo Cruz. 1986 Jan-Mar;81(1):61-6. doi: 10.1590/s0074-02761986000100008.
Twenty isolates of Schistosoma mansoni were obtained from patients, all males 13 to 30 years old, autochtonous from the Village of Capitão Andrade, municipality of Itanhomi, state of Minas Gerais, where an evolutive study of Mansoni's schistosomiasis is being performed since 1973. The isolates came from six patients with schistosome-infections (type I), six with the hepato-intestinal form (type II), and eight with the hepatosplenic form (type III). Each isolate was inoculated in mice divided in three groups of 16, exposed to 25, 50 and 100 cercariae, respectively. Controls were 12 uninfected mice. After 90 days adult worms were recovered from the portal by perfusion, from four mice of each experimental groups. Mice dying at various intervals and half of the rest f mice in each group sacrificed on the 90th and the 180th days were studied according to the following parameters: weight of liver, spleen, lung and intestine; egg count in the small intestine (proximal and medial) and large intestine (distal). The average numbers of worms obtained by perfusion from groups I, II and III were 21.9%, 22% and 17.8% respectively. The average natural mortality rates of mice submitted to infection with 25, 50 and 100 cercariae were, respectively, 12.4%, 23.2% and 40.2% for group I, 4.7%, 19.5% and 22.2% for group II and 11.4%, 29.5% and 41.6% for group III, being therefore proportional to the inocula.(ABSTRACT TRUNCATED AT 250 WORDS)
从患者身上获取了20株曼氏血吸虫分离株，所有患者均为13至30岁的男性，来自米纳斯吉拉斯州伊塔诺米市卡皮唐·安德拉德村的本地居民，自1973年以来一直在该地对曼氏血吸虫病进行一项演进性研究。这些分离株来自6例血吸虫感染患者（I型）、6例肝肠型患者（II型）和8例肝脾型患者（III型）。将每株分离株接种到分成三组、每组16只的小鼠体内，分别让它们接触25只、50只和100只尾蚴。对照组为12只未感染的小鼠。90天后，通过灌注从每个实验组的4只小鼠门静脉中回收成虫。对在不同时间段死亡的小鼠以及每组在第90天和第180天处死的其余一半小鼠，根据以下参数进行研究：肝脏、脾脏、肺和肠道的重量；小肠（近端和中段）和大肠（远端）中的虫卵计数。通过灌注从I组、II组和III组获得的虫体平均数量分别为21.9%、22%和17.8%。感染25只、50只和100只尾蚴的小鼠的平均自然死亡率，I组分别为12.4%、23.2%和40.2%，II组分别为4.7%、19.5%和22.2%，III组分别为11.4%、29.5%和41.6%，因此与接种量成正比。（摘要截断于250字）
