Conceição Maria José, Lenzi Henrique Leonel, Coura José Rodrigues
Department of Preventive Medicine, Infectious and Parasitic Diseases Post-Graduation, Clementino Fraga Filho Hospital, Universidade Federal do Rio de Janeiro, UFRJ, Rio de Janeiro, Brazil.
Acta Trop. 2008 Nov-Dec;108(2-3):98-103. doi: 10.1016/j.actatropica.2008.05.007. Epub 2008 May 21.
Twenty Schistosoma mansoni strains were isolated from three groups of patients (intestinal, hepatointestinal and hepatosplenic clinical forms) born and living in the town of Capitão Andrade, Minas Gerais, Brazil. Schistosomal isolate from each group was inoculated into three sets of mice with 25, 50 and 100 cercariae. The animals were killed 90 and 180 days after infection and submitted to extensive histopathological study of the liver, lung, intestine and spleen to determine qualitative and quantitative morphological characteristics, mainly of the granulomas. The histopathological changes caused the same patterns of infection in mice and were proportional to the inoculum and the time of infection, confirming the relevance of quantitative aspects in the determination of the disease. These data indicate three possibilities: (1) mouse model is not adequate to predict possible differences in the S. mansoni isolates obtained from patients; (2) field isolates are probably genetic polymorphic and undifferentiated; (3) schistosomiasis in human does not depend on parasite intrinsic factors, but on multivariable factors, such as intensity and duration of infection, time of infection, age and gender and other characteristics such as host response.
从出生并生活在巴西米纳斯吉拉斯州安德拉德船长镇的三组患者（肠道、肝肠和肝脾临床类型）中分离出20株曼氏血吸虫菌株。将每组的血吸虫分离株分别接种到三组小鼠体内，接种量分别为25、50和100尾尾蚴。在感染后90天和180天处死动物，并对肝脏、肺、肠道和脾脏进行广泛的组织病理学研究，以确定定性和定量的形态学特征，主要是肉芽肿的特征。组织病理学变化在小鼠中引起相同的感染模式，并且与接种量和感染时间成正比，证实了定量方面在疾病判定中的相关性。这些数据表明三种可能性：（1）小鼠模型不足以预测从患者获得的曼氏血吸虫分离株中可能存在的差异；（2）现场分离株可能是遗传多态且未分化的；（3）人类血吸虫病不取决于寄生虫的内在因素，而是取决于多变量因素，如感染强度和持续时间、感染时间、年龄和性别以及其他特征，如宿主反应。
