Baltimore R S, Fick R B, Fino L
Pediatr Res. 1986 Nov;20(11):1085-90. doi: 10.1203/00006450-198611000-00005.
The sera from 32 patients with cystic fibrosis who were chronically colonized with mucoid strains of Pseudomonas aeruginosa (MPA) were tested for anti-MPA antibodies. Using an enzyme-linked immunosorbent assay we measured IgA, IgG, and IgM antibody titers to three MPA strains, extracts of those strains, and seaweed-derived sodium alginate, which is similar chemically to the exopolysaccharide of MPA. These titers were compared with identical tests performed on the sera of eight cystic fibrosis patients who never were colonized with MPA and 10 normal adults. The IgG titers were significantly higher in tests of sera from the colonized patients compared with the other two groups but the IgA and IgM titers were not significantly higher. In colonized patients antibody titers to the different antigens correlated with each other suggesting that the major antibody response was to common antigenic determinants. Using these titers as a data base, eight patients whose clinical status was unknown to the testers, had IgG-enzyme-linked immunosorbent assay tests of their sera and the four colonized patients with cystic fibrosis were correctly identified. Three of them had substantial titers of antibody in the bronchoalveolar lavage fluid.
对32例长期被黏液型铜绿假单胞菌（MPA）定植的囊性纤维化患者的血清进行了抗MPA抗体检测。我们采用酶联免疫吸附测定法，检测了针对三种MPA菌株、这些菌株的提取物以及海藻衍生的海藻酸钠（其化学结构与MPA的胞外多糖相似）的IgA、IgG和IgM抗体滴度。将这些滴度与对8例从未被MPA定植的囊性纤维化患者以及10名正常成年人的血清进行的相同检测结果进行比较。与其他两组相比，定植患者血清检测中的IgG滴度显著更高，但IgA和IgM滴度并无显著升高。在定植患者中，针对不同抗原的抗体滴度相互关联，这表明主要的抗体反应针对的是共同的抗原决定簇。以这些滴度作为数据库，对8例检测者不知其临床状况的患者的血清进行了IgG酶联免疫吸附测定检测，其中4例囊性纤维化定植患者被正确识别。他们中有3人的支气管肺泡灌洗液中存在大量抗体滴度。
