Minoprio P M, Eisen H, Forni L, D'Imperio Lima M R, Joskowicz M, Coutinho A
Scand J Immunol. 1986 Dec;24(6):661-8. doi: 10.1111/j.1365-3083.1986.tb02185.x.
Lymphoid activity was studied in spleen and lymph node cells from Trypanosoma cruzi-infected mice. Blast transformation in each lymphocyte class was assessed by dual parameter analysis for size and surface markers by both FACS and conventional immunofluorescence, while proliferative activity was measured by tritiated thymidine uptake, autoradiography, and analysis of DNA content in single cells. Acute infection results in rapid blast transformation and proliferative activity of all three lymphocyte classes (Ig+, L3T4+, and Lyt 2+). At 2 weeks of infection most cells in these organs are enlarged and more than half are dividing. By 2 and 6 months after infection (chronic phase of resistant strains), large numbers of activated B lymphocytes and, to a lesser extent, of Lyt 2+ T cells are still detected. Similar results were obtained in C57BL/6 (resistant) and C3H/HeJ (susceptible) mouse strains. The implications of this massive polyclonal lymphocyte response to the parasite for the physiopathology of acute and chronic infection are discussed.
研究了克氏锥虫感染小鼠脾脏和淋巴结细胞中的淋巴细胞活性。通过FACS和传统免疫荧光对每种淋巴细胞类别的大小和表面标志物进行双参数分析来评估其母细胞转化，同时通过氚标记胸腺嘧啶核苷摄取、放射自显影和单细胞DNA含量分析来测量增殖活性。急性感染导致所有三种淋巴细胞类别（Ig +、L3T4 +和Lyt 2 +）迅速发生母细胞转化和增殖活性。感染2周时，这些器官中的大多数细胞增大，超过一半的细胞正在分裂。感染后2个月和6个月（抗性品系的慢性期），仍可检测到大量活化的B淋巴细胞，以及数量较少的Lyt 2 + T细胞。在C57BL / 6（抗性）和C3H / HeJ（易感）小鼠品系中获得了类似的结果。讨论了这种针对寄生虫的大规模多克隆淋巴细胞反应对急性和慢性感染生理病理学的影响。
