采用高压液相色谱法定量测定人血清和尿液中抗炎药芬布芬及其代谢产物。

Quantitation of the anti-inflammatory agent fenbufen and its metabolites in human serum and urine using high-pressure liquid chromatography.

作者信息

Van Lear G E, Chiccarelli F S, Bonenfant P A, Barr A

出版信息

J Pharm Sci. 1978 Dec;67(12):1662-4. doi: 10.1002/jps.2600671206.

Abstract

Specific procedures are described for the determination of fenbufen and its metabolites in serum and urine using high-pressure liquid chromatography. Serum or urine extracts were chromatographed on a bounded reversed-phase partitioning column. The sensitivity of the assay for fenbufen was 0.5 microgram/ml in serum with 2-ml samples and 1.0 microgram/ml in urine with 1-ml samples. The procedures are suitable for bioavailability and pharmacokinetic studies.

摘要

描述了使用高压液相色谱法测定血清和尿液中芬布芬及其代谢物的具体步骤。血清或尿液提取物在键合反相分配柱上进行色谱分析。对于芬布芬，该检测方法在血清中2毫升样品时的灵敏度为0.5微克/毫升，在尿液中1毫升样品时的灵敏度为1.0微克/毫升。这些步骤适用于生物利用度和药代动力学研究。