Vårdal Linda, Øiestad Elisabeth L, Gjelstad Astrid, Jensen Henrik, Pedersen-Bjergaard Stig
Department of Pharmacy, University of Oslo, PO Box 1068, Blindern, 0316 Oslo, Norway.
Department of Forensic Sciences, Oslo University Hospital, PO Box 4950 Nydalen, N-0424 Oslo, Norway.
Bioanalysis. 2019 Apr;11(8):755-771. doi: 10.4155/bio-2018-0308. Epub 2019 Apr 17.
Electromembrane extraction (EME) of 37 drugs of abuse with significant differences in terms of polarity (0.68 < log P < 4.3) and basicity (1.17 < pK < 10.38) was investigated from human plasma. EME was performed with 250 mM trifluoroacetic acid and DMSO (1:1 v/v) in the acceptor solution. The analytes were extracted efficiently with pure 2-nitrophenyloctyl ether as supported liquid membrane when the acceptor solution was modified with DMSO. Thus, using DMSO mixed with 250 mM trifluoroacetic acid (1:1, v/v) as acceptor solution, recoveries from 40 to 105% (relative standard deviation <20%) were obtained for 33 of the analytes under optimized conditions. EME followed by ultra-HPLC-MS/MS analysis was evaluated from human plasma, and the results were satisfactory.
研究了从人血浆中对37种滥用药物进行电膜萃取（EME）的情况，这些药物在极性（0.68 < log P < 4.3）和碱性（1.17 < pK < 10.38）方面存在显著差异。在接受液中使用250 mM三氟乙酸和二甲基亚砜（1:1 v/v）进行EME。当接受液用二甲基亚砜改性时，以纯2-硝基苯基辛醚作为支撑液膜可有效萃取分析物。因此，使用与250 mM三氟乙酸（1:1，v/v）混合的二甲基亚砜作为接受液，在优化条件下，33种分析物的回收率为40%至105%（相对标准偏差<20%）。对人血浆进行EME后再进行超高效液相色谱-串联质谱分析，结果令人满意。
