Genco Francesca, Sarasini Antonella, Parea Maurizio, Prestia Martina, Scudeller Luigia, Meroni Valeria
SC Microbiologia e Virologia Fondazione IRCCS Policlinico San Matteo Pavia, Italy.
Servizio di biometria e statistica Fondazione IRCCS Policlinico San Matteo Pavia, Italy.
New Microbiol. 2019 Apr;42(2):88-93. Epub 2019 Apr 17.
This study compared the performance of the LIAISON®XL system of immunoglobulin (Ig) G and IgM immunoassays for the diagnosis of Toxoplasma gondii, cytomegalovirus (CMV), and rubella virus infections with that of the ARCHITECT system. Patient serum samples, previously screened and clinically diagnosed with T. gondii, CMV or rubella, were used to compare LIAISON®XL and ARCHITECT IgG and IgM immunoassays. LIAISON®XL Toxo and CMV IgG avidity assays were also compared with equivalent ARCHITECT assays and reference methods. Overall agreement between the LIAISON®XL and ARCHITECT assays was 99% and 92% for the Toxo IgG and IgM assays, respectively, 98% and 96% for the CMV IgG and IgM assays, respectively, and 93% and 98% for the rubella virus IgG and IgM assays, respectively. LIAISON®XL IgG Toxo and CMV avidity assays showed high concordance with the VIDAS® Toxo IgG avidity assay and an in-house CMV avidity assay (reference methods), and faster IgG avidity maturation in a larger number of samples collected months after the primary infection compared with equivalent ARCHITECT assays. LIAISON®XL assays for detection of anti-T. gondii, CMV and rubella virus IgG and IgM are at least equal to the competitor assays on the ARCHITECT platform.
本研究比较了LIAISON®XL系统免疫球蛋白（Ig）G和IgM免疫测定法在诊断弓形虫、巨细胞病毒（CMV）和风疹病毒感染方面与ARCHITECT系统的性能。使用先前经筛查并临床诊断为弓形虫、CMV或风疹的患者血清样本，比较LIAISON®XL和ARCHITECT IgG及IgM免疫测定法。还将LIAISON®XL弓形虫和CMV IgG亲和力测定法与相应的ARCHITECT测定法及参考方法进行了比较。LIAISON®XL和ARCHITECT测定法在弓形虫IgG和IgM测定中的总体一致性分别为99%和92%，在CMV IgG和IgM测定中分别为98%和96%，在风疹病毒IgG和IgM测定中分别为93%和98%。LIAISON®XL弓形虫和CMV IgG亲和力测定法与VIDAS®弓形虫IgG亲和力测定法及一种内部CMV亲和力测定法（参考方法）显示出高度一致性，并且与相应的ARCHITECT测定法相比，在初次感染数月后采集的大量样本中，IgG亲和力成熟更快。LIAISON®XL检测抗弓形虫、CMV和风疹病毒IgG及IgM的测定法至少与ARCHITECT平台上的竞争测定法相当。
