Lykov A P, Poveshchenko O V, Surovtseva M A, Bondarenko N A, Kim I I, Kretov E I, Prokhorikhin A A, Tarkova A R, Malaev D U, Boĭkov A A
National Medical Research Centre named after Academician E.N. Meshalkin under the RF Ministry of Public Health, Novosibirsk, Russia; Research Institute of Clinical and Experimental Lymphology - Branch of the Institute of Pathology and Genetics under the Siberian Branch of the Russian Academy of Sciences, Novosibirsk, Russia.
National Medical Research Centre named after Academician E.N. Meshalkin under the RF Ministry of Public Health, Novosibirsk, Russia.
Angiol Sosud Khir. 2019;25(1):25-30. doi: 10.33529/angio2019103.
Vascular grafts made of polytetrafluoroethylene and polyethylene terephthalate have widely been used in cardiovascular surgery. The causes of delayed colonization of such grafts by endotheliocytes and mesenchymal stem cells have not been adequately investigated. The authors examined the effect of polyethylene terephthalate on the functional activity of human bone marrow mesenchymal stem cells and endothelial progenitor cells in vitro. Proliferation (MTT assay, real-time cellular impedance), migration (Boyden chamber, real-time cellular impedance), and nitric oxide production (spectrophotometciacally) by progenitor endothelial cells and mesenchymal stem cells were assessed with and without the presence of polyethylene terephthalate. The functional activity of the cells was shown to depend on the presence of polyethylene terephthalate in a well with cells. Thus, polyethylene terephthalate turned out to exhibit a toxic effect on progenitor endothelial and mesenchymal stem cells. Treatment of grafts with gelatine or fibronectin improved colonization of grafts with cells.
由聚四氟乙烯和聚对苯二甲酸乙二酯制成的血管移植物已广泛应用于心血管外科手术。内皮细胞和间充质干细胞对这类移植物延迟定植的原因尚未得到充分研究。作者在体外研究了聚对苯二甲酸乙二酯对人骨髓间充质干细胞和内皮祖细胞功能活性的影响。在有和没有聚对苯二甲酸乙二酯存在的情况下，评估祖内皮细胞和间充质干细胞的增殖（MTT 法、实时细胞阻抗）、迁移（Boyden 小室、实时细胞阻抗）以及一氧化氮生成（分光光度法）。结果表明，细胞的功能活性取决于培养孔中聚对苯二甲酸乙二酯的存在。因此，聚对苯二甲酸乙二酯对祖内皮细胞和间充质干细胞具有毒性作用。用明胶或纤连蛋白处理移植物可改善细胞对移植物的定植。
