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聚丙烯/聚对苯二甲酸乙二醇酯共混纤维增强胶原海绵中骨髓间充质干细胞的增殖和分化。

Proliferation and differentiation of mesenchymal stem cell on collagen sponge reinforced with polypropylene/polyethylene terephthalate blend fibers.

机构信息

Polymer Group, Department of Chemical Engineering, Tarbiat Modares University, Tehran, Iran.

出版信息

Tissue Eng Part A. 2010 Dec;16(12):3821-30. doi: 10.1089/ten.TEA.2009.0520. Epub 2010 Oct 8.

Abstract

Although tissue-engineered scaffolds made from collagen sponge are suitable for cell infiltrating, easily supplying oxygen and nutrients to cells, and removing the waste products, their mechanical properties are not satisfactory to be used as scaffold materials for tissue engineering applications. To improve mechanical properties of collagen, a novel porous scaffold for bone tissue engineering was prepared with collagen sponge reinforced by polypropylene/polyethylene terephthalate (PP/PET) fibers. Collagen solution (6.33 mg/mL) with PP/PET fibers (collagen/fiber ratio [w/w]: 1.27, 0.63, 0.42, 0.25) was freeze-dried, followed by cross-linking of combined dehydrothermal and glutaraldehyde. A scanning electron microscopy-based analysis of surface of the sponges demonstrated that the sponge with collagen/fiber <0.25 exhibited homogenous and interconnected pore structure with an average pore size of 200 μm. Incorporation of PP/PET fibers significantly enhanced the compressive strength of the collagen sponge. Proliferation and osteogenic differentiation of mesenchymal stem cell in collagen sponges reinforced with PP/PET fibers incorporation were significantly enhanced compared with collagen sponge without PP/PET incorporation. We conclude that incorporation of PP/PET fibers not only improves the mechanical properties of collagen sponge, but also enables mesenchymal stem cells to positively improve their proliferation and differentiation.

摘要

尽管由胶原海绵制成的组织工程支架适合细胞渗透,容易向细胞提供氧气和营养物质,并清除废物,但它们的机械性能并不令人满意,无法用作组织工程应用的支架材料。为了提高胶原的机械性能,用聚丙烯/聚对苯二甲酸乙二醇酯 (PP/PET) 纤维增强胶原海绵制备了一种用于骨组织工程的新型多孔支架。将胶原溶液(6.33mg/mL)与 PP/PET 纤维(胶原/纤维比[w/w]:1.27、0.63、0.42、0.25)混合,然后进行冷冻干燥,再进行联合去水热和戊二醛交联。对海绵表面进行基于扫描电子显微镜的分析表明,胶原/纤维<0.25 的海绵具有均匀且相互连通的孔结构,平均孔径为 200μm。PP/PET 纤维的掺入显著提高了胶原海绵的压缩强度。与未掺入 PP/PET 纤维的胶原海绵相比,掺入 PP/PET 纤维增强的胶原海绵中间充质干细胞的增殖和成骨分化明显增强。我们得出结论,PP/PET 纤维的掺入不仅提高了胶原海绵的机械性能,而且还使间充质干细胞能够积极地促进其增殖和分化。

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