Herron R, Bell A, Poole J, Clark M, Smith D S, Hamblin T J
Vox Sang. 1986;51(4):314-7. doi: 10.1111/j.1423-0410.1986.tb01975.x.
The serum of an 85-year-old Caucasian male with no history of blood transfusion contained an IgG3 antibody with anti-LWab specificity. The antibody failed to react with dithiothreitol-treated red cells, and there was a marked reduction in titre of the antibody with pronase-treated cells, findings consistent with an antibody having this specificity. High association values were obtained in a mononuclear phagocyte assay when LW-positive red cells, sensitised in vitro with the patient's serum antibody, were incubated with peripheral blood monocytes from the patient. In vivo red cell survival studies demonstrated that 99mTc-labelled rhesus-negative (rr), LW-positive red cells had 53% survival at 1 h. The IgG subclass of the antibody, mononuclear phagocyte assay results and in vivo survival studies predicted a significant reduction in the posttransfusion survival of therapeutic volumes of rhesus-negative (rr), LW-positive red cells.
一名85岁无输血史的高加索男性血清中含有一种具有抗-LWab特异性的IgG3抗体。该抗体与经二硫苏糖醇处理的红细胞不发生反应，且经链霉蛋白酶处理的细胞抗体效价显著降低，这些发现与具有这种特异性的抗体一致。当用患者血清抗体体外致敏的LW阳性红细胞与患者外周血单核细胞一起孵育时，在单核吞噬细胞试验中获得了高结合值。体内红细胞存活研究表明，99mTc标记的恒河猴阴性（rr）、LW阳性红细胞在1小时时的存活率为53%。抗体的IgG亚类、单核吞噬细胞试验结果和体内存活研究预测，治疗量的恒河猴阴性（rr）、LW阳性红细胞输血后存活率将显著降低。
