Rice W C, Miller L K
Virus Res. 1986 Nov;6(2):155-72. doi: 10.1016/0168-1702(86)90047-x.
Regulation of transcription within four well-defined regions of the DNA genome of the baculovirus Autographa californica nuclear polyhedrosis virus (AcNPV) was studied in a permissive lepidopteran cell line. Spodoptera frugiperda IPLB-SF-21 and a nonpermissive dipteran cell line, Drosophila melanogaster DL-1. Cycloheximide, an inhibitor of protein synthesis, was used to identify immediate early transcripts and aphidicolin, an effective inhibitor of DNA replication, was used to distinguish early and late transcripts in S. frugiperda cells. Immediate early transcripts were identified in the HindIII-K (85 to 87.5%), the HindIII-I/EcoRI-F (I/F from 35 to 37%) and the HindIII-P/EcoRI-P (p10 from 88.5 to 89.5%) regions of the genome. Late RNAs, defined by their sensitivity to aphidicolin as well as cycloheximide, overlapped the immediate early RNAs. Specific viral transcripts were synthesized in nonpermissive D. melanogaster cells infected with AcNPV but late viral transcripts were not observed.
在一种允许的鳞翅目细胞系中研究了苜蓿银纹夜蛾核型多角体病毒（AcNPV）DNA基因组四个明确区域内的转录调控。使用了草地贪夜蛾IPLB-SF-21细胞系和一种非允许的双翅目细胞系——黑腹果蝇DL-1细胞系。蛋白质合成抑制剂放线菌酮用于鉴定即刻早期转录本，DNA复制有效抑制剂阿非迪霉素用于区分草地贪夜蛾细胞中的早期和晚期转录本。在基因组的HindIII-K（85%至87.5%）、HindIII-I/EcoRI-F（I/F为35%至37%）和HindIII-P/EcoRI-P（p10为88.5%至89.5%）区域鉴定出了即刻早期转录本。根据其对阿非迪霉素和放线菌酮的敏感性定义的晚期RNA与即刻早期RNA重叠。在用AcNPV感染的非允许黑腹果蝇细胞中合成了特定的病毒转录本，但未观察到晚期病毒转录本。
