School of Chemical Engineering , Northeast Electric Power University , Jilin 132012 , China.
Division of Biotechnology , Dalian Institute of Chemical Physics , CAS, Dalian 116023 , China.
Org Lett. 2019 May 3;21(9):3218-3222. doi: 10.1021/acs.orglett.9b00935. Epub 2019 Apr 17.
An Escherichia coli strain was constructed for the efficient import of nicotinamide adenine dinucleotide (NAD) analogues into cells by limiting extracellular degradation while expressing an efficient NAD importer. In vivo functions of three NAD analogues were characterized. Nicotinamide hypoxanthine dinucleotide was identified as an inhibitor of NAD synthesis. Nicotinamide cytosine dinucleotide had excellent biocompatibility and was used for characterizing a growth-dependent degradation of in vivo nicotinamide cofactors.
构建了一株大肠杆菌菌株，通过限制细胞外降解来提高烟酰胺腺嘌呤二核苷酸（NAD）类似物向细胞内的导入效率，同时表达高效的 NAD 导入蛋白。对三种 NAD 类似物的体内功能进行了表征。烟酰胺次黄嘌呤二核苷酸被鉴定为 NAD 合成的抑制剂。烟酰胺胞苷二核苷酸具有极好的生物相容性，并用于表征体内烟酰胺辅因子的生长依赖性降解。
