Abrahám M, Kirstein D, Scheller F, Boross L
Acta Biochim Biophys Hung. 1986;21(3):225-8.
A coupled enzymatic method elaborated for NAD+-dependent dehydrogenases has been adapted for NADP+-dependent isocitrate dehydrogenase, in combination with amperometric measurements. The isocitrate dehydrogenase activity dependent linearly on the isocitrate concentration in the range 0-2 X 10(-4) M. Application of this method affords a sensitive estimation of isocitrate even in turbid liquids such as fermentation broths.
一种为依赖NAD +的脱氢酶精心设计的偶联酶法已被改编用于依赖NADP +的异柠檬酸脱氢酶，并结合了安培测量法。异柠檬酸脱氢酶的活性在0 - 2×10（-4）M范围内与异柠檬酸浓度呈线性关系。即使在诸如发酵液等浑浊液体中，应用该方法也能对异柠檬酸进行灵敏的测定。
