Raunio R P, Lövgren T N, Kurkijärvi K
Anal Biochem. 1985 Nov 1;150(2):315-9. doi: 10.1016/0003-2697(85)90516-0.
A bioluminescent assay for NADPH-dependent isocitrate dehydrogenase and for its substrates and cofactors was developed. The method is based on continuous NADPH monitoring in the reaction. The linear range of the assay for the enzyme activity is from 0.05 U/liter to 30 U/liter. It is about 300 times more sensitive than the corresponding spectrophotometric assay at 340 nm. Good correlation exists between both assays. Isocitrate, NADP, manganese, and magnesium can be measured at picomole levels. The applicability of the assays to serum analysis is discussed.
开发了一种用于NADPH依赖性异柠檬酸脱氢酶及其底物和辅因子的生物发光测定法。该方法基于反应中对NADPH的连续监测。酶活性测定的线性范围为0.05 U/升至30 U/升。它比相应的340 nm分光光度法灵敏约300倍。两种测定法之间存在良好的相关性。异柠檬酸、NADP、锰和镁可以在皮摩尔水平进行测定。讨论了这些测定法在血清分析中的适用性。
