Department of Histology and Embryology, Pomeranian Medical University in Szczecin, Powst. Wlkp. 72, 70-111 Szczecin, Poland.
Department of Nephrology, Transplantology and Internal Medicine, Pomeranian Medical University in Szczecin, Powst. Wlkp. 72, 70-111 Szczecin, Poland.
Int J Environ Res Public Health. 2019 Apr 16;16(8):1370. doi: 10.3390/ijerph16081370.
Immunosuppressive drugs are widely used to avoid graft rejection, but they are also known to be strongly hepatotoxic. The goal of the current study was to determine: (i) the immunoexpression of SOD1, CAT, GPX1; (ii) the concentration of MDA, GSH; (iii) the activity of SOD, CAT, GPX, in the native liver of a pregnant female rats undergoing immunosuppressive therapy. The study was based on archival material obtained from Department of Nephrology, Transplantology and Internal Medicine of the Independent Public Clinical Hospital No. 2 at the Pomeranian Medical University in Szczecin, Poland. The study was carried out on 32 female rats exposed to oral administration of immunosuppressants two weeks before and during pregnancy. The percentage of SOD1 immunopositive hepatocytes in rats treated with cyclosporine A, mycophenolate mofetil, everolimus, and glucocorticosteroid was significantly elevated above that of the control rats. The concentration of MDA in the liver of animals exposed to cyclosporine A, everolimus, and glucocorticosteroid was significantly higher than in other groups. Among the groups of dams treated with immunosuppressive drugs, the highest significant concentration of GSH was found in the livers of rats treated with cyclosporine A, mycophenolate mofetil and glucocorticosteroid. Immunosuppressive therapy during pregnancy affects the oxidoreductive balance in the livers of rats, depending on the regimen used.
免疫抑制剂广泛用于避免移植物排斥反应，但它们也被认为具有很强的肝毒性。本研究的目的是确定：(i) SOD1、CAT、GPX1 的免疫表达；(ii) MDA、GSH 的浓度；(iii) 接受免疫抑制治疗的妊娠雌性大鼠的天然肝脏中的 SOD、CAT、GPX 活性。该研究基于波兰什切青波美拉尼亚医科大学独立公共临床医院 2 号肾脏病学、移植学和内科的档案材料。该研究在 32 只雌性大鼠中进行，这些大鼠在怀孕前两周和怀孕期间接受了口服免疫抑制剂治疗。用环孢素 A、霉酚酸酯、依维莫司和糖皮质激素治疗的大鼠中 SOD1 免疫阳性肝细胞的百分比明显高于对照组大鼠。暴露于环孢素 A、依维莫司和糖皮质激素的动物肝脏中 MDA 的浓度明显高于其他组。在接受免疫抑制剂治疗的母体大鼠组中，用环孢素 A、霉酚酸酯和糖皮质激素治疗的大鼠肝脏中发现了最高的 GSH 浓度。怀孕期间的免疫抑制治疗会影响大鼠肝脏的氧化还原平衡，具体取决于所使用的方案。
