Clark D G, Neville S D, Brinkman M, Nelson P V, Illman R J, Guthberlet A, Haynes W D
Biochem J. 1986 Sep 15;238(3):811-6. doi: 10.1042/bj2380811.
The effects of food deprivation on body weight, liver weight, hepatic glycogen content, glycogenolytic enzymes and blood metabolites were compared in young and old phosphorylase b kinase-deficient (gsd/gsd) rats. Although the concentration of glycogen in liver from 9-week-old female gsd/gsd rats (730 mumol of glucose equivalents/g wet wt.) was increased by 7-8% during starvation, total hepatic glycogen was decreased by 12% after 24 h without food. In 12-month-old male gsd/gsd rats the concentration of liver glycogen (585 mumol of glucose equiv./g wet wt.) was decreased by 16% and total hepatic glycogen by nearly 40% after food deprivation for 24 h. Phosphorylase b kinase and phosphorylase a were present at approx. 10% of the control activities in 9-week-old gsd/gsd rats, but both enzyme activities were increased more than 3-fold in 12-month-old affected rodents. It is concluded that the age-related ability to mobilize hepatic glycogen appears to result from the augmentation of phosphorylase b kinase during maturation of the gsd/gsd rat.
在年轻和年老的磷酸化酶b激酶缺陷（gsd/gsd）大鼠中，比较了食物剥夺对体重、肝脏重量、肝糖原含量、糖原分解酶和血液代谢物的影响。尽管9周龄雌性gsd/gsd大鼠肝脏中的糖原浓度（730微摩尔葡萄糖当量/克湿重）在饥饿期间增加了7-8%，但在禁食24小时后，肝脏总糖原减少了12%。在12月龄雄性gsd/gsd大鼠中，禁食24小时后，肝糖原浓度（585微摩尔葡萄糖当量/克湿重）降低了16%，肝脏总糖原降低了近40%。在9周龄的gsd/gsd大鼠中，磷酸化酶b激酶和磷酸化酶a的活性约为对照活性的10%，但在12月龄的患病啮齿动物中，这两种酶的活性均增加了3倍以上。得出的结论是，gsd/gsd大鼠成熟过程中磷酸化酶b激酶的增加似乎导致了与年龄相关的肝糖原动员能力。
