马属动物肉瘤表面拭子和刮片中牛乳头瘤病毒DNA的聚合酶链反应检测
PCR detection of bovine papilloma virus DNA in superficial swabs and scrapings from equine sarcoids.
作者信息
Martens A, De Moor A, Ducatelle R
机构信息
Department of Large Animal Surgery and Anaesthesiology, Ghent University, Salisburylaan, Belgium.
出版信息
Vet J. 2001 May;161(3):280-6. doi: 10.1053/tvjl.2000.0524.
The purpose of this study was to examine if bovine papilloma virus (BPV) DNA can be detected in superficial swabs or scrapings from equine sarcoids. Samples were obtained from 92 sarcoids and 20 non-sarcoidal control lesions. The polymerase chain reaction technique was used with a first primer set to check whether DNA extraction was successful, and with a second primer set specific for BPV-DNA. DNA isolation was successful in 88% of the swabs and 93% of the scrapings. All control lesions were negative for BPV-DNA.
本研究的目的是检测马肉瘤表面拭子或刮片中是否能检测到牛乳头瘤病毒（BPV）DNA。从92个肉瘤和20个非肉瘤对照病变中获取样本。采用聚合酶链反应技术，用第一组引物检查DNA提取是否成功，并用第二组针对BPV-DNA的特异性引物进行检测。88%的拭子和93%的刮片中DNA分离成功。所有对照病变的BPV-DNA检测均为阴性。
Zotero 插件