Kaleczyc J, Kasica-Jarosz N, Pidsudko Z, Przyborowska A, Sienkiewicz W
Department of Animal Anatomy, Faculty of Veterinary Medicine, University of Warmia and Mazury, Oczapowskiego 13, 10-718 Olsztyn, Poland.
Pol J Vet Sci. 2019 Mar;22(1):151-155. doi: 10.24425/pjvs.2019.127081.
The present study investigated the expression of androgen receptor (AR) in neurons of the anterior pelvic ganglion (APG) and celiac-superior mesenteric ganglion (CSMG; ganglion not involved in the innervation of reproductive organs) in the male pig with quantitative real-time PCR (qPCR) and immunohistochemistry. qPCR investigations revealed that the level of AR gene expression in the APG tissue was approximately 2.5 times higher in the adult (180-day-old) than in the juvenile (7-day-old) boars. Furthermore, in both the adult and juvenile animals it was sig- nificantly higher in the APG than in CSMG tissue (42 and 85 times higher, respectively). Immu- nofluorescence results fully confirmed those obtained with qPCR. In the adult boars, nearly all adrenergic (DβH-positive) and the majority of non-adrenergic neurons in APG stained for AR. In the juvenile animals, about half of the adrenergic and non-adrenergic neurons were AR-posi- tive. In both the adult and juvenile animals, only solitary CSMG neurons stained for AR. The present results suggest that in the male pig, pelvic neurons should be considered as an element of highly testosterone-dependent autonomic circuits involved in the regulation of urogenital func- tion, and that their sensitization to androgens is a dynamic process, increasing during the prepu- bertal period.
本研究采用定量实时聚合酶链反应（qPCR）和免疫组织化学方法，研究雄激素受体（AR）在雄性猪的盆前神经节（APG）和腹腔-肠系膜上神经节（CSMG；该神经节不参与生殖器官的神经支配）神经元中的表达。qPCR研究显示，成年（180日龄）公猪APG组织中AR基因表达水平比幼年（7日龄）公猪高约2.5倍。此外，在成年和幼年动物中，APG组织中的AR基因表达水平均显著高于CSMG组织（分别高42倍和85倍）。免疫荧光结果完全证实了qPCR的结果。在成年公猪中，APG中几乎所有肾上腺素能（多巴胺β羟化酶阳性）神经元和大多数非肾上腺素能神经元均有AR染色。在幼年动物中，约一半的肾上腺素能和非肾上腺素能神经元为AR阳性。在成年和幼年动物中，只有单个CSMG神经元有AR染色。目前的结果表明，在雄性猪中，盆神经节神经元应被视为参与泌尿生殖功能调节的高度依赖睾酮的自主神经回路的一个组成部分，并且它们对雄激素的敏感性是一个动态过程，在青春期前会增加。
