Skwarcz S, Bryzek I, Gregosiewicz A, Warda E, Gawęda K, Tarczyńska M, Węgłowski R, Skwarcz J, Nadulski R, Starek A, Sanford J
Veterinary Clinic - Bryzek and Partners, Lublin, Poland.
Department of Paediatric Orthopaedics, Medical University, Lublin, Poland.
Pol J Vet Sci. 2019 Mar;22(1):109-115. doi: 10.24425/pjvs.2019.127077.
Platelet-rich plasma (PRP) preparations can be used in bone tissue healing but there are numerous doubts among clinical orthopedists about effectiveness of this method.
The studies were carried out in 12 rabbits of white termond breed. In operating room we operationally generated cylindrical, unicortical defects of the diameter of 4 mm in the middle of the shafts of both femurs. The defects in the left bones were left without filling and served as controls, and 0.7 ml of the ready-to-use PRP was administered to the defects in the right bones (experimental group). We evaluated the usefulness of the diagnostic methods applied: biomechanical tests, micro-CT tests, densitometry, typical radiology, macroscopic measurements, histopathological examinations.
The macroscopic measurements showed a statistically significant increase in the dimension in the area of the right defect filled with PRP in relation to the control group. In experimented group, the assessment of the X-ray images showed the formation of a callus cuff around the defects. Densitometric examinations showed no statistically significant differences between defects in the experimental and control group. The analysis of the micro-CT examina- tions showed an increase in the total volume of the tissue examined (Vb) and the low density tissue fraction (Vb2) in the experimental group. The biomechanical examinations revealed signi- ficant decrease in the maximum breaking force (F max) necessary to break the bone in the experi- mental group in relation to the control group.
Platelet-rich plasma (PRP) stimulates bone formation in the area of bone defects and may accelerate bone regeneration.
富血小板血浆（PRP）制剂可用于骨组织愈合，但临床骨科医生对该方法的有效性存在诸多疑问。
对12只白色新西兰兔进行研究。在手术室中，在双侧股骨骨干中部手术制造直径为4毫米的圆柱形单皮质骨缺损。左侧骨缺损不进行填充作为对照，右侧骨缺损注入0.7毫升即用型PRP（实验组）。我们评估了所应用诊断方法的有效性：生物力学测试、显微CT测试、骨密度测定、传统放射学、宏观测量、组织病理学检查。
宏观测量显示，与对照组相比，注入PRP的右侧缺损区域尺寸在统计学上有显著增加。在实验组中，X线图像评估显示缺损周围形成了骨痂套。骨密度检查显示实验组和对照组的缺损之间无统计学显著差异。显微CT检查分析显示实验组中所检查组织的总体积（Vb）和低密度组织部分（Vb2）增加。生物力学检查显示，与对照组相比，实验组中使骨断裂所需的最大断裂力（Fmax）显著降低。
富血小板血浆（PRP）可刺激骨缺损区域的骨形成，并可能加速骨再生。
