不同冷冻保存能力的公猪精子中的总RNA质量

Total RNA quality in boar spermatozoa with different freezability.

作者信息

Fraser L, Brym P, Mogielnicka-Brzozowska M, Wasilewska K

机构信息

Department of Animal Biochemistry and Biotechnology, Faculty of Animal Bioengineering, University of Warmia and Mazury in Olsztyn, 10-719 Olsztyn, Poland.

Department of Animal Genetics, Faculty of Animal Bioengineering, University of Warmia and Mazury in Olsztyn, 10-719 Olsztyn, Poland.

出版信息

Pol J Vet Sci. 2019 Mar;22(1):181-185. doi: 10.24425/pjvs.2019.127085.

DOI:10.24425/pjvs.2019.127085

PMID:30997782

Abstract

In this study the quality of total RNA, isolated from fresh spermatozoa, was compared between boars with good and poor semen freezability (GSF and PSF, respectively). Semen from 3 boars with GSF exhibited significantly higher total motility, mitochondrial function, plasma membrane integrity and reduced lipid peroxidation compared with 3 boars with PSF after cryo- preservation. There were variations in the quality of RNA isolated from spermatozoa of boars with GSF and PSF. Boars with GSF exhibited mainly full-length, intact RNA, whereas substantial amounts of degraded RNA were detected in spermatozoa from boars with PSF. Further under- standing of the biological relevance of RNAs in sperm function is critical to improve the freezabil- ity of boar semen.

摘要

在本研究中，对分别具有良好和较差精液冷冻能力（分别为GSF和PSF）的公猪新鲜精子中分离出的总RNA质量进行了比较。与3头具有较差精液冷冻能力的公猪相比，3头具有良好精液冷冻能力的公猪精液在冷冻保存后表现出显著更高的总活力、线粒体功能、质膜完整性以及更低的脂质过氧化水平。从具有良好和较差精液冷冻能力的公猪精子中分离出的RNA质量存在差异。具有良好精液冷冻能力的公猪主要表现为全长、完整的RNA，而在具有较差精液冷冻能力的公猪精子中检测到大量降解的RNA。进一步了解RNA在精子功能中的生物学相关性对于提高公猪精液的冷冻能力至关重要。